Phenotypic Character and HPLC Fingerprints of Radial Striations from Different Germplasms Rehmanniae Radix
10.13422/j.cnki.syfjx20191214
- VernacularTitle: 不同种质地黄块根菊花心表型性状及HPLC指纹图谱特征分析
- Author:
Jian-guang ZHU
1
;
Feng-qing WANG
2
;
Cai-xia XIE
1
;
Xiao-yu GUO
1
;
Miao ZHANG
1
;
Xiao-sai TAO
1
;
Xiao-tong GENG
1
;
Jing-wei LEI
1
;
Zhong-yi ZHANG
3
Author Information
1. Henan University of Chinese Medicine, Zhengzhou 450046, China
2. Henan Agricultural University, Zhengzhou 450002, China
3. Fujian Agriculture and Forestry University, Fuzhou 350021, China
- Publication Type:Research Article
- Keywords:
Rehmanniae Radix;
radial striation;
phenotypic character;
HPLC fingerprint;
principal component analysis;
systematic cluster analysis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(12):166-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To discuss the phenotypic character and the HPLC fingerprints of radial striations from different germplasms Rehmanniae Radix. Method: The changes in the shape and column diameter of the radial striations of Rehmanniae Radix were observed and measured in the whole growth period. Besides,the HPLC fingerprints of the root,radial and un-radial striations were established to sign the chemical quality and analyzed by principal component analysis(PCA)and systematic cluster analysis. Result: There were significantly differences and regularities in the shape and proportion of the radial striations of different germplasms Rehmanniae Radix. The fingerprints showed the consistency between different types of chemical ingredients,and the differences in chemical quality characteristics mainly lay in the content of chemical compositions and theirs relative ratio. The results of PCA indicated that active ingredients, such as acteoside,catalpol,rehmaionoside D,rehmaionoside A and leonuride, were involved in the quality expression of different parts from various germplasms of Rehmanniae Radix,but each ingredient had a distinctive contribution rate to the differential quality expression between different parts from various germplasms of Rehmanniae Radix. However,the other components involved in the differential quality expression had different contribution rates in different germplasms.The systematic cluster analysis indicated that great differences in the chemical quality between the radial striations and un-radial striations of Beijing-1,Qinhuai,Qinhuai Zheng and 1706 germplasms,but with small differences in 85-5 and Baixuan germplasms. Conclusion: There are differences in phenotypic character of the radial striations and HPLC fingerprints between different germplasms Rehmanniae Radix.
