Effect of Rosae Chinensis Flos Total Flavones on Focal Cerebral Ischemia-reperfusion in Rats
10.13422/j.cnki.syfjx.20190804
- VernacularTitle: 月季花总黄酮对局灶性脑缺血再灌注模型大鼠的影响
- Author:
Yan LI
1
;
Ya-gang SONG
2
;
Ming-san MIAO
2
;
Shuai SHAO
2
Author Information
1. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
2. Henan University of Chinese Medicine, Zhengzhou 450046, China
- Publication Type:Research Article
- Keywords:
Rosae Chinensis Flos total flavones (RCTF);
middle cerebral artery acclusion(MCAO) model;
cerebral ischemia;
reperfusion injury
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(12):64-70
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of Rosae Chinensis Flos total flavones(RCTF) on the focal cerebral ischemia-reperfusion model in rats, in order to preliminarily explore the mechanism of action. Method: Rats were randomly divided into sham-operated group, model group, large, medium, and low-dose RCTF group(200,100,50 mg ·kg-1) and positive group[Nimodipine group(20 mg ·kg-1) and Naoluotong group (500 mg ·kg-1)]. After 7 days of continuous administration, 1 hour later after the last administration, the middle cerebral artery middle cerebral artery occlusion (MCAO) model was duplicated. After 2 hours of modeling, perfusion was performed for 22 hours. Mortality and neurological deficits were scored. Serum S-100β was detected; brain tissue malondialdehyde(MDA), superoxide dismutase (SOD), nitric oxide (NO), nitric oxide synthase (NOS), tumour necrosis factor-α(TNF-α), interleukin-1β(IL-1β), intercellular adhesion molecule-1(ICAM-1), adenosine triphosphate (ATP)ase were measured. The brain tissue morphological changes were observed. Result: The rat model of focal cerebral ischemia and reperfusion was successfully replicated. Compared with the model group, RCTF in large, medium, and low-dose RCTF group significantly decreased the score of neurological deficit in rats (P<0.01), significantly decreased the content of S-100β in serum (P<0.01), significantly reduce the levels of MDA, NO and NOS in brain tissue (P<0.01), significantly increase the level of SOD in brain tissue (P<0.01), and significantly increase Na+K+-ATPase, Mg2+-ATPase, and Ca2+ in brain tissue (P<0.01), significantly reduced TNF-α content, IL-1β, ICAM-1 content in brain tissue (P<0.01), and significantly improved brain tissue damage (P<0.01). Conclusion: RCTF have a protective effect on cerebral ischemia-reperfusion injury in rats. The mechanism may be related to the resistance of anti-free radicals, the reduction of inflammation in brain tissue and the improvement of brain energy metabolism after cerebral ischemia reperfusion injury.
