Chemical Constituents from Stems of Zanthoxylum bungeanum
10.13422/j.cnki.syfjx.20191113
- VernacularTitle: 花椒茎的化学成分
- Author:
Jing-rong LOU
1
;
Zhong-fei ZHENG
2
;
Ying LI
2
;
Chao QI
1
;
Xue CUI
1
;
Qing-qiang YAO
2
Author Information
1. School of Medicine and Life Sciences, University of Ji'nan-Shandong Academy of Medical Sciences, Ji'nan 250200, China
2. Institute of Materia Medica, Shandong Academy of Medical Sciences, Key Laboratory for Biotech-Drugs Under Ministry of Health, Key Laboratory for Rare & Uncommon Diseases of Shandong Province, Ji'nan 250062, China
- Publication Type:Research Article
- Keywords:
Zanthoxylum bungeanum stems;
flavone;
alkaloid;
coumarin;
triterpene
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(11):161-166
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate and identify the chemical constituents from 95%ethanol extracts from stems of Zanthoxylum bungeanum. Method:The stems (25 kg) were extracted with 95%ethanol under reflux for three times and then filtrated,and the combined filtrates were concentrated under vacuum to get the extracts. After suspension with water,the extracts were extracted with petroleum ether,dichloromethane,ethyl acetate and n-butanol successively,and five corresponding parts were obtained. The parts of petroleum ether,dichloromethane,ethyl acetate were separated in a similar way to get pure compounds. By the method the subjects were first chromatographed on silica gel column respectively,then the selected sub-fractions were further separated by Sephadex LH-20, and finally purified on preparation HPLC to get the monomer compound. The n-butanol part was first treated with macroporous resin D101,and then the sub-fractions were further purified by almost the same method as mentioned above. The structures of these compounds were determined by spectrum technology as IR,MS,1H-NMR,13 C-NMR. Result:Ten compounds were isolated from Z. bungeanum stems and identified as follows:(+)-magnoflorine(1),(-)-marmesin(2),(-)-columbianetin(3),(-)-decursinol(4),lupeol(5),α-amyrin(6),β-amyrin(7),δ-amyrin(8),quercetin(9),rutin(10). Among them, 1 was alkaloid, 2,3,4 were coumarin, 5,6,7,8 were triterpenoid, 9,10 were flavonoids. Conclusion:Compounds 1-8 were isolated from this plant for the first time.
