Effect of Changji'an Prescription on Intestinal Permeability of IBS-D Rats
10.13422/j.cnki.syfjx.20190905
- VernacularTitle: 肠激安方对IBS-D大鼠肠道通透性的影响
- Author:
Yu-ying SHI
1
;
Hong-mei TANG
2
;
Ying-xiu WU
1
;
He ZHU
1
;
Guo-dong HE
1
;
Fen XIONG
2
;
Yi-jing ZHANG
1
Author Information
1. Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
2. The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
- Publication Type:Research Article
- Keywords:
Changji'an prescription;
diarrhea-predominant irritable bowel syndrome(IBS-D);
intestinal permeability;
tight junction protein;
Sijunzi Tang;
Tongxie Yaofang
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(11):80-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Changji'an prescription on intestinal permeability in diarrhea-predominant irritable bowel syndrome (IBS-D) rats and explore its mechanism for treatment of IBS-D. Method:Male SD neonatal rats were randomly divided into five groups:normal group, model group, pinaverium bromide group(0.018 g·kg-1), high-dose(33.48 g·kg-1) and low-dose (16.74 g·kg-1)Changji'an prescription groups. Except for the normal group, the IBS-D model was established by the combination of maternal and infant separation+acetic acid stimulation+restraint stress. After drug treatment, the ultrastructure of rat intestinal mucosa was observed by using transmission electron microscopy and the plasma D-lactate level was detected by enzyme-linked immunosorbent assay (ELISA). The expression levels of tight junction proteins Occludin and Claudin-1 were detected by Western blot. The mRNA expression levels of Occludin,Claudin-1 and zonula occluden(ZO)-1 were detected by real time polymerase chain reaction (Real-time PCR). Result:As compared with the normal group, the intestinal mucosal epithelial cells were damaged in IBS-D model group, and the microvilli arrangement was sparse and tight junction was widened, and some were not obvious,and the plasma D-lactate level in IBS-D rats was increased significantly (P<0.05),and the mRNA and protein expression levels of Occludin and Claudin-1 in the colon were decreased and the mRNA expression of ZO-1 was also decreased (P<0.05). After drug treatment, as compared with the model group, all drug-administered groups can repair intestinal mucosal cell damage. Plasma D-lactate level in pinaverium bromide group and high-dose Changji'an prescription group was significantly decreased (P<0.05) while D-lactate level in the low-dose group Changji'an prescription group had a tendency to decrease with no statistical difference. The mRNA and protein expression levels of Occludin and Claudin-1 and the mRNA expression of ZO-1 in the colon of rats in each administration group were higher than those in the model group (P<0.05). Conclusion:The therapeutic effect of Changji'an prescription on IBS-D may be achieved by improving the intestinal permeability.
