Effect of Different Administration Methods with Bleomycin on Pulmonary Fibrosis in Rats
10.13422/j.cnki.syfjx.20191104
- VernacularTitle: 博来霉素不同给药方式致大鼠肺纤维化模型探讨
- Author:
He WANG
1
;
Guang-ping ZHANG
2
;
Hong-ping HOU
2
;
Yun-hang GAO
2
;
Shuang-rong GAO
2
;
Meng MA
2
;
Hai-jing ZHANG
2
;
Zhong-xiu ZHANG
2
;
Teng-fei CHEN
2
;
Ping SU
2
;
Ling SONG
2
;
Yi-fei YANG
2
;
Jiang LI
1
;
Zu-guang YE
2
Author Information
1. Guiyang University of Traditional Chinese Medicine, Guiyang 550002, China
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
bleomycin;
pulmonary fibrosis model;
aerosol inhalation;
hydroxyproline;
plasminogen activator inhibitor-1
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(11):73-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Compare the effects of 3 administration methods (tracheal perfusion, tail vein injection and aerosol inhalation) with bleomycin (BLM) in inducing pulmonary fibrosis in rats, in order to find out the optimal administration methods. Method:Eighty sprague-dawley (SD) male rats with SPF were randomly divided into aerosol inhalation blank group, single tracheal perfusion group(10 mg·kg-1), multiple tracheal perfusion group(5 mg·kg-1), single intravenous injection group(150 mg·kg-1), multiple intravenous injection group(50 mg·kg-1), single aerosol inhalation group (30 min)and multiple aerosol inhalation group(30 min). The mortality and body weight of rats in each group were observed at 7 d, 14 d and 28 d after the administration. And 28 days later after the administration, the lung coefficients of rats in each group were observed, paraffin sections were prepared, hematoxylin-eosin staining (HE) and Masson staining were performed, and the contents of hydroxyproline (HYP) and plasminogen activator inhibitor-1 (PAI-1) in lung tissues were detected by enzyme-linked immunosorbent assay (ELISA), so as to evaluate the alveoli inflammation and pulmonary fibrosis of rats in each group. Result:Compared with the aerosol inhalation blank group, the rats in the trachea perfusion group had the highest mortality among the drug treatment groups. The pulmonary coefficients of rats in the multiple intravenous injection group and the multiple inhalation group were significantly higher than those in the blank group(P<0.05,P<0.01). The multiple inhalation group was higher than the other model group and the single atomization model group. The results of HE and Masson staining showed thickening of pulmonary septum and higher degree of pulmonary interstitial fibrosis in tracheal perfusion group, intravenous injection group and multiple inhalation group. The degree of pulmonary fibrosis in the multiple inhalation group was more obvious than that in other groups. The results of ELISA showed that the levels of HYP and PAI-1 in lung tissues of rats in aerosol inhalation group and tracheal perfusion group were significantly higher than those in control group(P<0.05). The multiple inhalation group and the single atomization inhalation group were significantly higher than other modules. Conclusion:Bleomycin was inhaled repeatedly to establish pulmonary fibrosis model. The pathological injury and physiological indexes of the model rats were relatively stable, which conforms with the evolution process of pulmonary fibrosis.
