Effect of Tanreqing Injection Combined with Imipenem-cilastatin in Inhibiting Extensively-drug Resistant Pseudomonas aeruginosa
10.13422/j.cnki.syfjx.20191107
- VernacularTitle: 痰热清注射液与亚胺培南西司他丁联合对广泛耐药铜绿假单胞菌的抑菌作用
- Author:
Ya-jun XING
1
;
Wei-feng YANG
1
;
Ya-nan SUN
1
;
Shu-hua MA
1
;
Tong-tong JIANG
2
;
Gai-ying HE
1
;
Xiao-li ZHANG
3
;
Yi WANG
1
Author Information
1. Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. Jilin Agricultural University, Changchun 130118, China
3. Shanghai Kaibao Pharmaceutical Co. Ltd., Shanghai 201401, China
- Publication Type:Research Article
- Keywords:
Tanreqing;
imipenem-cilastatin;
Pseudomonas aeruginosa;
synergistic effect;
biofilm;
planktonic bacteria
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(11):60-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of combination of Tanreqing injection(Tanreqing) and imipenem-cilastatin on extensively-drug resistant Pseudomonas aeruginosa (XDPA), and study the mechanism of the combination. Method:The minimum inhibitory concentrations (MICs) of Tanreqing and imipenem-cilastatin against planktonic XDPA strain isolated in clinic were determined by the broth microdilution method. The checkerboard method was used to evaluate the combination effect. The bacterial metabolic activity in mature biofilm was studied by microtiter-plate test. The destructive effect of combination drugs on dynamic biofilm was observed by using BioFlux system, and viable cells were examined by confocal laser scanning microscope (CLSM) after treatment. The scanning electron microscopy (SEM) was used for observing Pseudomonas aeruginosa and length measurement. Result:The MIC values of imipenem-cilastatin and Tanreqing were 512 mg·L-1 and more than 16 500 mg·L-1. The checkerboard analysis showed that Tanreqing could enhance the sensitivity of imipenem-cilastatin, while the combination drugs synergistically inhibited the growth of bacteria. Compared with the control group or the imipenem-cilastatin individual group, the combined drugs significantly reduced the amount of living bacteria in the biofilm (P<0.05,P<0.01). BioFlux results showed that the combination drugs destructed the biofilm structure and reduced the area coverage (P<0.05) by comparing with the control group or the single drug group. The results of fluorescent staining showed that the combination drug significantly inhibited the metabolic activity of bacteria in dynamic biofilm. Tanreqing inhibited bacterial division to achieve the antibacterial effect. Conclusion:Tanreqing and imipenem-cilastatin synergistically inhibit the bacterial growth in planktonic and biofilm states, and destruct biofilms.
