Effect of Gancao Fuzitang on Synovial Fibroblast-like Cell Proliferation in Adjuvant Arthritis Mice Model
10.13422/j.cnki.syfjx.20191003
- VernacularTitle: 甘草附子汤对佐剂性关节炎小鼠滑膜成纤维样细胞增殖的影响
- Author:
Yue CAI
1
;
Bo ZHANG
1
;
Jing GUO
1
Author Information
1. First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
- Publication Type:Research Article
- Keywords:
Gancao Fuzitang;
adjuvant arthritis;
rheumatoid arthritis;
fibroblast-like synoviocytes;
proliferation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(11):29-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Gancao Fuzitang on the proliferation of fibroblast-like synoviocytes (FLS) in synovial tissue of mice with adjuvant-induced arthritis (AA). Method:The 48 male Balb/c mice were randomly divided into 4 groups:normal control group, model group(arthritis induced through injection with adjuvant), Gancao Fuzitang group and triptolide group. The normal control group and the model control group were orally given physiological saline. After the successful modeling, the mice of the other two groups were treated with Gancao Fuzitang or triptolide orally for 18 days. Then the animals were euthanized, the paw edema of the animals was measured, and arthritic ankles were observed by histological examination. Tumor necrosis factor (TNF)-α was examined by immunoassays. The proliferation of synovial cells of foot joints was detected by immunohistochemical expressions of Vimentin. The expressions of Cyclin D1, proliferating cell nuclear antigen (PCNA), p53 and p21 were detected by Western blot. Result:Compared with the normal control group, the mice of synovial hyperplasia and bone erosion in model group were obvious. The joint swelling degree, TNF-α level and proliferation of FLS were increased (P<0.05). The expressions of Cyclin D1,PCNA were up-regulated(P<0.05), but the expressions of p53 and p21 were down-regulated(P<0.05). Compared with the model group, Gancao Fuzitang significantly alleviated joint swelling and injuries,reduced the level of TNF-α, cell cycles (Cyclin D1 and PCNA), and increased protein expressions of p53 and p21 (P<0.05). Conclusion:Gancao Fuzitang has a therapeutic effect on AA mice, and the mechanism might be associated with its anti-inflammatory effect and the effects in regulating Cyclin D1, PCNA, p53 and p21 expressions and inhibiting FLS proliferation.
