Regulatory Effect of Sijunzi Tang and Its Single Herbs on Intestinal Flora in Rats with Spleen Deficiency
10.13422/j.cnki.syfjx.20190847
- VernacularTitle: 四君子汤及其单味药水煎液对脾虚大鼠肠道菌群的调节作用
- Author:
Wen-wu HUANG
1
;
Ying PENG
1
;
Meng-yue WANG
1
;
Chong-sheng PENG
1
;
Xiao-bo LI
1
Author Information
1. School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China
- Publication Type:Research Article
- Keywords:
Sijunzi Tang;
spleen deficiency;
intestinal flora;
short-chain fatty acids;
diversity index;
similarity index;
polymerase chain reaction
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(11):8-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulatory effect of Sijunzi Tang(SJZT) and its single herbs(Ginseng Radix et Rhizoma, Glycyrrhizae Radix et Rhizoma Praeparata cum Melle, Atractylodis Macrocephalae Rhizoma and Poria) on intestinal flora in spleen-deficient rats. Method:Normal rats were randomly divided into the blank group, model group, Zhengchangsheng granules group, SJZT group and each single herb group, rats were orally administered Sennae Folium decoction to induce diarrhea for ten consecutive days to establish a spleen-deficient model(distilled water for the blank group), then treated with the corresponding drugs for seven consecutive days(distilled water for the blank group and the model group). Fresh feces were collected on pre-modeling(0th day), post-modeling(11th day), and post-treatment(18th day). Short-chain fatty acids(SCFAs) in feces were acidified by sulphuric acid and extracted by diethyl ether, then determined by gas chromatography. The structural change(diversity and similarity) of intestinal flora in feces was analyzed by 16S rDNA-polymerase chain reaction(PCR)-denaturing gel gradient electrophoresis(DGGE) technique. Result:Compared with blank group, the contents of SCFAs as well as diversity and similarity indexes of intestinal flora in feces of all administered groups were significantly decreased on the 11th day(P<0.05, P<0.01), this indicated that the spleen-deficient model was successfully constructed. On the 18th day, compared with model group, the contents of SCFAs as well as diversity and similarity indexes of intestinal flora in feces of Atractylodis Macrocephalae Rhizoma group were significantly increased(P<0.01), these indexes in feces of SJZT group, Ginseng Radix et Rhizoma group and Poria group were significantly increased(P<0.05, P<0.01), while Glycyrrhizae Radix et Rhizoma Praeparata cum Melle group only increased diversity index of intestinal flora(P<0.05). Conclusion:Ginseng Radix et Rhizoma, Atractylodis Macrocephalae Rhizoma and Poria are the single herbs responsible for the regulatory effect of SJZT on intestinal flora in spleen-deficient rats, and Atractylodis Macrocephalae Rhizoma may play a major role.
