Effect of Astragalus Polysaccharide on Sweet Taste Receptor Pathway in Intestine of Rat Model Induced by High-sugar and High-fat Diet
10.13422/j.cnki.syfjx.20191037
- VernacularTitle: 黄芪多糖对高糖高脂饮食模型大鼠肠道甜味受体通路的影响
- Author:
Ying WANG
1
;
Si-yu CHEN
1
;
Ze-min YANG
1
Author Information
1. College of Life Sciences and Biopharmaceuticals of Guangdong Pharmaceutical University, Guangzhou 510006, China
- Publication Type:Research Article
- Keywords:
high-sucrose and high-fat diet;
sweet taste receptor pathway;
astragalus polysaccharides;
proglucagon gene;
taste receptor 1 member 2/taste receptor 1 member 3
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(10):64-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of astragalus polysaccharide (APS) on taste receptor 1 member 2 (T1R2)/taste receptor 1 member 3 (T1R3) sweet taste receptor pathway in intestine of rat model induced by high-sugar and high-fat diet. Method:SD rats were randomly divided into normal group, high-sugar and high-fat group and astragalus polysaccharide group. Rats in high-sugar and high-fat group and astragalus polysaccharide groups were fed with high-sugar and high-fat diet for 16 weeks, while rats in astragalus polysaccharide group were fed with APS (0.7 g·kg-1, per day) for 8 weeks during this period. Serum samples were collected to determine the levels of fasting blood glucose, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C). Intestinum tenue was collected to determine mRNA expressions of T1R2/T1R3, α-gustducin (Gα gust), transient receptor potential cation channel subfamily member 5 (TRPM5) and proglucagon (PG) gene by Real-time PCR, and protein expressions of T1R2, Gα gust and glucagon-like peptide-1 (GLP-1) protein by Western blot. Result:Rats in high-sugar and high-fat group had significantly higher levels of TC, TG and LDL-C, and lower HDL-C level in serum than those in normal group (P<0.05). Moreover, the expression levels of sweet receptor pathway molecules, including T1R2, Gα gust and PG genes in intestine, were significantly down-regulated in high-sugar and high-fat group (P<0.05). Rats in astragalus polysaccharide group had significantly lower levels of TC, TG and LDL-C, and higher HDL-C level in serum than those in high-sugar and high-fat group (P<0.05). The expression levels of T1R2, T1R3, Gα gust, TRPM5 and PG genes in intestine were significantly up-regulated in astragalus polysaccharide group (P<0.05). The trend of T1R2, Gα gust and GLP-1 protein expressions was consistent with that of T1R2, Gα gust and GLP-1 mRNA expressions. Protein expressions of T1R2, Gα gust and GLP-1 and mRNA expression of T1R3 were significantly lower in astragalus polysaccharide group than those of control group (P<0.05). Conclusion:APS could improve disturbance of lipid metabolism and impairment of intestinal sweet taste receptor pathway for rat model induced by high-sugar and high-fat diet.
