Effect of Zhizhuwan on Intestinal Conduction and PLC-γ1/PLC-γ2 Signaling Pathway of Slow Transit Constipation Model Mouse with Spleen Deficiency Syndrome
10.13422/j.cnki.syfjx.20191039
- VernacularTitle: 枳术丸对脾虚证慢传输型便秘小鼠肠道运动及PLC-γ1/PLC-γ2信号通路的影响
- Author:
Wei-song WANG
1
;
Xu-ting XIA
2
;
Fu-lin LIU
2
;
Wen-feng WANG
3
;
Yu-bo CHEN
4
;
Guang-fei TENG
2
;
Ting WANG
1
;
Qin-rong TU
1
Author Information
1. The First Hospital of Hunan University of Chinese Medicine, Changsha 410000, China
2. College of Traditional Chinese Medicine of Hunan University of Chinese Medicine, Changsha 410000, China
3. Admission and Employment Office of Hunan University of Chinese Medicine, Changsha 410000, China
4. The Second Hospital of Hunan University of Chinese Medicine, Changsha 410000, China
- Publication Type:Research Article
- Keywords:
Zhizhuwan;
constipation;
slow transit constipation;
phospholipase C-γ1/phospholipase C-γ2 signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(10):8-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of Zhizhuwan on intestinal conduction and expressions of Phospholipase C-γ1 (PLC-γ1)/Phospholipase C-γ2 (PLC-γ2) signaling pathway of slow transit constipation (STC) with spleen deficiency syndrome. Method:Special pathogen free (SPF) healthy mice were randomly divided into normal group and model making group. Folium Sennae gavage was used to induce the spleen deficiency status, and then diet and drinking water were controlled to establish the mice model of spleen deficiency constipation. After the modeling, the mice in modelling group were randomly divided into model group, Zhizhuwan group and mosapride group. Zhizhuwan group was given drug at the dose of 9.0 g·kg-1·d-1, mosapride group was given 2.5 mg·kg-1·d-1, model group and normal group were given the equal dose of distilled water for 7 consecutive days. At the end of the treatment, the length of Indian ink in the colon was used to calculate the intestinal propulsion rate of the mice. The D-xylose kit was used to determine the content of D-xylose in serum of mice. The hematoxylin eosin (HE) staining was used to observe the pathological changes of colon tissues in mice. The immunohistochemistry and Western blot were used to detect the expression levels of PLC-γ1 and PLC-γ2 proteins in colon tissues of mice. Real-time PCR was used to detect the mRNA expression levels of PLC-γ1 and PLC-γ2. Result:Compared with normal group, the intestinal propulsive rate and the serum D-xylose value in model group were significantly decreased (P<0.01). Obvious hyperemia and edema of colonic mucosa were observed by HE staining. The protein and mRNA expression levels of PLC-γ1 and PLC-γ2 in colon tissues were significantly decreased (P<0.01). Compared with model group, the intestinal propulsion rate and the content of D-xylose in Zhizhuwan group and mosapride group were significantly increased (P<0.01). Colonic mucosal hyperemia, edema and inflammatory cell infiltration were improved according to HE staining. The protein and mRNA expression levels of PLC-γ1 and PLC-γ2 were significantly increased (P<0.01). Conclusion:Zhizhuwan can promote the intestinal movement in slow transit constipation model mouse with spleen deficiency syndrome, and alleviate the symptoms of constipation in mice. The related mechanism may be related to the increase of the expressions of PLC-γ1 and PLC-γ2 in colon tissues of mice with spleen deficiency and constipation.
