Screening and Identification of Endophytic Fungi in Scutellariae Radix Transformed Baicalin to Baicalein and Oroxylin A
10.13422/j.cnki.syfjx.20190105
- VernacularTitle: 黄芩苷发酵转化成黄芩素和千层纸素A的黄芩内生真菌的筛选及鉴定
- Author:
Zong-min MA
1
;
Jia LIU
1
;
Xu-hong DUAN
1
;
Guo-qiang SUN
1
;
Lu-qi HUANG
2
;
Lin PEI
1
Author Information
1. Hebei Key Laboratory of Turbiditytoxin Syndrome, Hebei Academy of Chinese Medical Sciences, Shijiazhuang 050031, China
2. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
baicalin;
baicalein;
oroxylin A;
Penicillium sp. R3;
Scutellariae Radix;
potato dextrose agar;
conversion rate
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(9):166-171
- CountryChina
- Language:Chinese
-
Abstract:
Objective: In order to explore natural active ingredients of Scutellariae Radix and alleviate the pressure of traditional medicinal resources,this research aimmed to screen endophytic fungus strains from Scutellariae Radix which could transform baicalin into some active ingredients.Method: Taking fresh plants of Scutellariae Radix as strain resource,endophytic fungus strains were isolated by potato dextrose agar (PDA) plate separation,Scutellariae Radix powder selective culture and HPLC detection.Result: An endophytic fungus strain R3 was isolated and it can transform baicalin to baicalein and oroxylin A simultaneously in potato dextrose broth (PDB) contained 0.1% baicalin and the conversion rate reached 61.09% in 5 d cultured at 28℃ and 150 r·min-1,molar ratio of baicalein and oroxylin A was 3:5.With the method of morphological analysis,microscopic identification and 18SrDNA sequence analysis,the bacterium was identified as Penicillium sp.R3. Conclusion: Penicillium sp.R3 can transform baicalin to baicalein and oroxylin A with certain research value and application value.
