Quality Evaluation System of Standard Decoction of Puerariae Thomsonii Radix
10.13422/j.cnki.syfjx.20190321
- VernacularTitle: 粉葛标准汤剂质量评价体系的建立
- Author:
Yan-lin ZHANG
1
;
Qing-hong MENG
1
;
Yi SUN
2
;
Zi-quan FAN
3
;
Dan-dan WANG
3
;
Er-bing HUA
1
Author Information
1. College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
3. Waters Technologies (Shanghai) Co. Ltd., Shanghai 201206, China
- Publication Type:Research Article
- Keywords:
Puerariae Thomsonii Radix;
quality evaluation method;
standard decoction;
puerarin;
transfer rate;
fingerprint
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(9):144-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the quality control methods for the standard decoction of Puerariae Thomsonii Radix.Method:According to the preparation principles for traditional Chinese medicine (TCM) standard decoction,13 batches of Puerariae Thomsonii Radix from different origins were analyzed under the chromatography conditions established in this study and verified with methodology.By referring to Chinese Pharmacopoeia of 2015,puerarin was used as a quantitative indicator to calculate the transfer rate.In this study,the structures of main chromatographic peaks were also identified to clarify the main chemical constituents in the standard decoction.Result:The 13 batches of medicinal herbs were identified as Puerariae Thomsonii Radix,with a recovery rate of 98.0%,and RSD of 1.1%,indicating that the method was accurate and reliable.The transfer rate ranged from 41.4% to 60.0%,and the extraction rate was within the range of 15.7%-34.3%.The corresponding fingerprints were prepared for 13 batches of the standard decoction,and their similarities were all greater than 90.0%.The chemical constituents from Puerariae Thomsonii Radix were identified by mass spectrometry analysis,including citric acid,4'-O-glucosyl puerarin/daidzein-4',7-diglucoside,3'-hydroxy puerarin/genistein puerarin,puerarin apioside,daidzin puerossid A and daidzein,etc.Conclusion: The 13 batches of Puerariae Thomsonii Radix decoction in different origins had consistent properties with the basic properties of medicinal decoction pieces.The established method of quality evaluation can be used to systematically evaluate the standard decoction,providing reference for quality control of related decoction preparations of Puerariae Thomsonii Radix.
