Analysis of Rhizosphere Soil Fungal Community Composition and Diversity of Salvia miltiorrhiza Based on ITS Sequences
10.13422/j.cnki.syfjx.20190715
- VernacularTitle: 基于ITS序列的丹参连作根际土壤真菌群落组成及多样性分析
- Author:
Wei LIU
1
;
Bing-qian ZHOU
1
;
Xiao WANG
1
;
Heng LU
1
;
Lan-ping GUO
2
;
Feng-sheng LI
3
Author Information
1. Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Shandong Key Laboratory of Traditional Chinese Medicine(TCM)Quality Control Technology, Ji'nan 250014, China
2. China Academy of Chinese Medical Sciences, National Resource Center for Chinese Materia Medica, Post-Doctoral Research Station of Chinese Academy of TCM, Beijing 100700, China
3. Laiwu Ziguang Ecological Company, Laiwu 271100, China
- Publication Type:Research Article
- Keywords:
Salvia miltiorrhiza;
continuous cropping;
high-throughtput sequencing;
fungal community;
diversity
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(9):130-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study rhizosphere soil fungal community composition and diversity of Salvia miltiorrhiza,in order to explain the changes of the rhizosphere microenvironment of S.miltiorrhiza,and provide theoretical basis for revealing the reasons for the formation of continuous obstacles.Method: Based on the high-throughput sequencing technology,three kinds of soil samples were collected,namely soil of non-plant (CK),soil for cultivating for one year (Y1) and soil for cultivating for two years (Y2),respectively.According to the sequencing of the internal transcribed space of nuclear ribosomal DNA (ITS) region of fungal rRNA gene,14 153,19 024 and 7 622 valid sequences were obtained and annotated as 1 027,1 095 and 712 varieties of OTUs.The diversity of fungal communities showed a trend of decreasing first and then increasing.Agaricomycetes,Leotiomycetes and Tremellomycetes were the dominant bacterial communities at the class level,their orders in three treatments were Y1 > CK > Y2,Y2 > Y1 > CK,CK > Y2 > Y1.At the genus level,Guehomyces was dominant species in CK,and both Y1 and Y2 had a decrease of 96.13% and 90.21%,respectively.Wickerhamomyces had a significantly increase in Y2(6.64%),250.59% and 564.00% compared with CK and Y1,respectively.Result: The results of cluster analysis showed that CK and Y1 had a higher similarity at the genus level in soil fungal community composition compared with Y2.Conclusion: Cryptosporiopsis and other pathogenic fungal communities were dominant genuses according to the heatmap analysis in Y2,suggesting a correlation with the incidence of S.miltiorrhiza.The findings provide theoretical basis for the formation of continuous cropping obstacle.
