Changes of Chemical Components in Raw Products and Characteristic Processed Products with Porcine Cardiac Blood of Salviae Miltiorrhizae Radix et Rhizoma from Menghe Medical School by UPLC-Q-TOF/MS
10.13422/j.cnki.syfjx.20190204
- VernacularTitle: UPLC-Q-TOF/MS分析孟河医派特色猪心血丹参炮制前后化学成分的变化
- Author:
Xiao-jing YAN
1
;
Bo-wen ZHENG
2
;
Yi-da ZHANG
2
;
Na LI
2
;
Chan-ming LIU
1
;
Zhen CAO
1
;
Qi ZHANG
1
Author Information
1. Menghe Medical Institute, Changzhou Affiliated Hospital of Nanjing University of Chinese Medicine, Changzhou 213003, China
2. State Key Laboratory of Quality Research of Chinese Medicine, Macau University of Science and Technology, Macau 999078, China
- Publication Type:Research Article
- Keywords:
Menghe medical school;
characteristic processing;
Salviae Miltiorrhizae Radix et Rhizoma processed with porcine cardiac blood;
water-soluble ingredients;
fat-soluble ingredients;
amino acids;
ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF/MS)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(9):109-116
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the chemical components in raw products and characteristic processed products with porcine cardiac blood of Salviae Miltiorrhizae Radix et Rhizoma from Menghe medical school.Method: The ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS) was performed on an ACQUITY UPLC® C18 column (2.1 mm×100 mm,1.7 μm),mobile phase was 0.1% formic acid aqueous solution (A)-0.1% formic acid acetonitrile solution (B) for gradient elution (0-1 min,8% B,1-1.5 min,8%-20% B,1.5-4 min,20% B,4-5 min,20%-60% B,5-9 min,60%-70% B,9-10 min,70%-95% B,10-13 min,95% B).The column temperature was 40℃ and the flow rate was 0.3 mL·min-1.Q-TOF/MS with electrospray ionization (ESI) and scanning range of m/z 50-1 200 were applied for analysis under positive and negative ion mode,respectively.All ionic peaks were assigned by comparison of reference substances,mass spectra data,database matching and literature reference,changes of chemical components in Salviae Miltiorrhizae Radix et Rhizoma were investigated by comparing number and area of ionic peaks before and after processing.Result: A total of 59 components were identified from raw products and characteristic processed products,and peak areas of 25 components showed obvious change.However,there were no new compound was found in characteristic processed products.After being processed with porcine cardiac blood,the contents of water-soluble ingredients (protocatechuic aldehyde,salvianolic acid C,F,G),fat-soluble ingredients (tanshinaldehyde,tanshindiol A,tanshinone Ⅰ) and amino acid (L-phenylalanine) in Salviae Miltiorrhizae Radix et Rhizoma were significantly increased.Conclusion: Changes of contents of chemical components in raw products and processed products of Salviae Miltiorrhizae Radix et Rhizoma are remarkable,part of water-soluble ingredients,fat-soluble ingredients and amino acids have quantitative change,which may be related to promoting treatment of Salviae Miltiorrhizae Radix et Rhizoma on cerebral ischemia after being processed with porcine cardiac blood.
