Effect and Mechanisms of Lycii Fructus on Hepatoma HepG2 Cell Line-induced Cachexia in Mice
10.13422/j.cnki.syfjx.20190924
- VernacularTitle: 鲜枸杞子提取物通过p38 MAPK信号通路抑制人肝癌细胞HepG2诱导小鼠恶病质的作用及机制
- Author:
Ting LIU
1
;
Yan DING
2
;
Ting-ting YANG
1
;
Xiao-na MA
1
;
Yi-ran JIN
1
;
Hai-bin MA
1
;
Xue-yun LIANG
1
Author Information
1. Ningxia Human Stem Cell Institute, General Hospital of Ningxia Medical University, Yinchuan 750004, China
2. General Hospital of Ningxia Medical University, Yinchuan 750004, China
- Publication Type:Research Article
- Keywords:
cachexia;
liver cancer;
HepG2;
pro-inflammatory cytokines;
Lycii Fructus
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(9):89-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the inhibitory effect and mechanism of the extracts from fresh Lycii Fructus (LBL) on hepatoma HepG2 cell-induced cachexia in mouse.Method: The human hepatoma cell line HepG2 was injected into BALB/C mice to establish the cachexia model.Then the LBL was fed to the models respectively in low dose (5 mg·kg-1) or high dose (25 mg·kg-1).After 28 days of continuous feeding,the mice's body weight was detected.The expression levels of creatine kinase (CK),interleukin-1β (IL-1β),interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) were evaluated by enzyme linked immunosorbent assay (ELISA).The muscle degradation and the expression of p38 mitogen-activated protein kinase (p38 MAPK) were detected by immunohistochemistry staining.The expression levels of phospho-p38 mitogen-activated protein kinase (p-p38 MAPK) and nuclear factor-κB (NF-κB) were determined by Western blot.Result: Compared with cachexia group,the loss of body weight and muscle decomposition were significantly inhibited both in the low dose LBL group and high dose LBL group (P<0.05,P<0.01).The level of plasma CK decreased significantly both in the low-dose LBL group and the high-dose LBL group (P<0.01).ELISA tests revealed lower expression levels of IL-1β,IL-6 and TNF-α in both the low-dose LBL group and the high-dose LBL group (P<0.05,P<0.01).Immunohistochemistry staining showed that the expression of p38 MAPK was inhibited both in the low-dose LBL group and the high-dose LBL group (P<0.05).Western blot indicated that the expressions of p-p38 MAPK and NF-κB were inhibited both in the low-dose LBL group and the high-dose LBL group (P<0.01).We found that the high-dose LBL group shows a higher inhibitory capability than the low-dose LBL group.Conclusion: LBL could inhibit the cachexia induced by hepatoma HepG2 cell line in mouse,suggesting LBL could reduce major cytokine and plasma inflammatory factors through p-p38 MAPK pathway.
