Mechanism of Prunellae Spica Extracts on Lipid Metabolism in ZDF Rats Based on AMPK/ACC Signalling Pathway
10.13422/j.cnki.syfjx.20190806
- VernacularTitle: 基于AMPK/ACC信号通路探讨夏枯草提取物调节ZDF大鼠脂代谢的机制
- Author:
Shuo TIAN
1
;
Li-li WU
1
;
Wei-hua ZHANG
2
;
Xiao-ke DONG
3
;
Yi-fan HAN
3
;
Ming-zhao HONG
4
Author Information
1. Health Cultivation Laboratory Under the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, China
2. Beijing Body Revival Medical Technology Co. Ltd., Beijing 100088, China
3. Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
4. Guangming New District People's Hospital, Shenzhen 518106, China
- Publication Type:Research Article
- Keywords:
Prunellae Spica extracts;
type 2 diabetes mellitus;
lipid metabolism;
insulin resistance;
AMP-activated protein kinase/acetyl CoA carboxylase(AMPK/ACC)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(9):82-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of Prunellae Spica extracts (PS) on the lipid metabolism in Zuker Diabetes Fatty (ZDF) rats based on AMP-activated protein kinase/acetyl CoA carboxylase (AMPK/ACC) signaling pathway.Method: The 32 male ZDF (fa/fa) type 2 diabetic rats were randomly divided into model group,metformin group (180 mg·kg-1·d-1),and low and high-dose PS groups (12.25,24.5 mg·kg-1·d-1),with 8 in each group.8 male Zuker Lean (ZL) rats were selected as normal group.Body weight and fasting blood glucose were monitored at the 0th,4th and 8th weeks after administration.After 8 weeks,abdominal aorta blood was collected,serum was frozen at-20℃ by centrifugation,liver tissue was frozen at-80℃,fixed with 4% paraformaldehyde and embedded in paraffin.Serum triglyceride (TG),cholesterol (CHO),low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA) levels were measured by radioimmunoassay.Fat droplets in hepatocytes were measured by oil red O staining.Gene expressions of AMP-activated protein kinase-alpha 2(AMPKα2),Acetyl CoA carboxylase (ACC) in liver were detected by real-time polymerase chain reaction (Real-time PCR).Protein expressions of p-AMPKα were observed by immuno-histochemical (IHC) method.Result: Compared with the normal group,the T2DM model group showed significant increases in serum levels of TG,CHO,LDL-C,FFA and lipid droplets in hepatocytes.AMPKα2 mRNA expression was decreased,while ACC1 and ACC2 mRNA expressions were increased significantly.p-AMPKα protein expression in liver was decreased significantly (P<0.05,P<0.01).Compared with the model group,low and high-dose Ps groups showed significant decreases in serum levels of TG,CHO,LDL-C,FFA and lipid droplets in hepatocytes in ZDF rats,up-regulation in mRNA expression of AMPKα2,down-regulation in mRNA expressions of ACC1 and ACC2,and up-regulation in protein expression of p-AMPKα(P<0.05,P<0.01).Conclusion:PS can effectively improve liver lipid metabolism in ZDF rats.Its mechanism may be related to the regulation of AMPK/ACC signaling pathway in liver.
