Effect of Modified Si Junzitang Drug Serum on Expression of Apoptosis-related Molecules of Gastric Cancer Cell SGC-7901
10.13422/j.cnki.syfjx.20190824
- VernacularTitle: 加味四君子汤含药血清对胃癌细胞SGC-7901凋亡相关因子表达的影响
- Author:
Shan-shan NIE
1
;
Xun LI
1
;
Yu-hang ZHAO
1
;
Dong-sheng WANG
1
Author Information
1. Xiangya Hospital, Central South University, Changsha 410008, China
- Publication Type:Research Article
- Keywords:
modified Si Junzitang (MSJZT) drug serum;
gastric cancer SGC-7901;
apoptosis;
tumor suppressor gene p53 (p53);
c-nucleoprotein gene (c-Myc);
cysteine-aspartic acid protease-3 (Caspase-3);
B-cell lymphoma-2 (Bcl-2)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(9):25-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of modified Si Junzitang (MSJZT) drug serum on the expression of apoptosis-related molecules of gastric cancer cell SGC-7901 and further its anti-tumor mechanism.Method: A total of 40 SD rats were randomly divided into four groups:low-dose,middle-dose,high-dose MSJZT (0.213,0.426,0.853 g·kg-1) groups and normal group (n=10).The treatment groups were administrated through gastric perfusion,and the normal group was given the equivalent volume of normal saline for 10 days.1.5 h after the last treatment,chloral hydrate peritoneal anesthesia was performed,blood was collected from heart,and different doses of serum were separated to prepare drug-containing serum of low-dose,middle-dose,high-dose MSJZT groups,in order to incubate SGC-7901 gastric cancer cell.Early and late apoptosis rates were detected with flow cytometry.Afterwards,the tumor suppressor gene p53,c-nucleoprotein gene (c-Myc),cysteine-aspartic acid protease-3(Caspase-3),B-cell lymphoma-2(Bcl-2) mRNA expressions were confirmed by fluorescence quantitative polymerase chain reaction (Real-time PCR).The protein expressions of p53,c-Myc,Caspase-3,Bcl-2 were detected by immunofluorescence.Result: Compared with the normal group,the high-dose MSJZT group could obviously increase the apoptosis rate to 22.58%(P<0.01).The results of Real-time PCR showed that the middle-dose MSJZT group significantly promoted the mRNA expression of Caspase-3(P<0.01),the medium-dose group significantly increased the mRNA expressions of p53 and c-Myc (P<0.01),and the high-dose group significantly inhibited the mRNA expression of Bcl-2(P<0.01).The immunofluorescence results showed that the high-dose MSJZT group could significantly increase the protein expressions of c-Myc,Caspase-3 and p53(P<0.01),while the protein expression of Bcl-2 was significantly reduced (P<0.05).Conclusion: MSJZT drug serum could exert an anti-tumor effect by inhibiting the expression of the anti-apoptotic protein Bcl-2,and promoting the expressions of pro-apoptotic-related molecules p53,c-Myc,Caspase-3.
