Effect of Duhuo Jisheng Tang on PERK/Bip Signaling Pathway in Knee Osteoarthritis Model Rats
10.13422/j.cnki.syfjx.20190937
- VernacularTitle: 独活寄生汤对膝骨关节炎模型大鼠PERK/Bip信号通路的影响
- Author:
Lin-can ZHAO
1
;
Wei-wei LI
2
;
Yi-ming WU
2
;
Wei-feng DUAN
3
Author Information
1. Zhengzhou Shuqing Medical College, Zhengzhou 450000
2. The Third Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450004, China
3. Luoyang Orthopedic-traumatological Hospital of Henan Province, Luoyang 471000, China
- Publication Type:Research Article
- Keywords:
Duhuo Jisheng Tang;
osteoarthritis;
protein kinase R-like endoplasmic reticulum kinase(PERK);
immunoglobulin-binding protein(Bip);
signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(9):18-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the possible mechanism of Duhuo Jisheng Tang in relieving knee osteoarthritis based on protein kinase R-like endoplasmic reticulum kinase (PERK)/immunoglobulin-binding protein (Bip) signaling pathway.Method: A model of knee osteoarthritis was established by cold stimulation.Rats were randomly divided into blank group,model group,celecoxib group (0.021 g·kg-1),low,medium and high-dose Duhuo Jisheng Tang groups (8.37,16.72,33.48 g·kg-1).Blank group and model group were given equal volume of physiological saline.The changes of knee joint diameter were recorded.The pathological changes of rat articular cartilage were observed by hematoxylin-eosin (HE) staining.The expressions of tumor necrosis factor-alpha (TNF-α),interleukin-1β(IL-1β) and hyaluronic acid (HA) in serum were detected by enzyme-linked immunosorbent assay (ELISA).The mRNA and protein expression levels of PERK,Bip and cysteinyl as parates pecific protein-9(Caspase-9) in cartilage were detected by Real-time PCR and Western blot.Result: The knee joint redn ess and the joint diameter of celecoxib group and high-dose Duhuo Jisheng Tang group were improved,and the joint diameter was reduced significantly (P<0.01).The local fractures of knee joint cartilage in the celecoxib group and the high-dose Duhuo Jisheng Tang group were significantly improved.Compared with blank group,the expression levels of TNF-α,IL-1β and HA were increased in model group (P<0.01).The gene and protein expression levels of PERK,Bip and Caspase-9 in cartilage of the model group were increased (P<0.05,P<0.01).Compared with model group,the expressions of TNF-α,IL-1β and HA in serum of celecoxib group and high-dose Duhuo Jisheng Tang group were decreased (P<0.05,P<0.01).The mRNA and protein expression levels of PERK,Bip and Caspase-9 in cartilage of celecoxib group and high-dose Duhuo Jisheng Tang group were decreased (P<0.05,P<0.01).Conclusion: Duhuo Jisheng Tang can alleviate the symptoms of knee osteoarthritis model rats,and its mechanism may be related to the regulation of PERK/Bip signaling pathway in rat cartilage.
