Hypoglycemic Mechanism of Ziya Biti Tablet Based on LC-MS Metabolomics Technique
10.13422/j.cnki.syfjx.20190604
- VernacularTitle: 基于LC-MS的代谢组学技术分析孜亚比提片的降糖作用机制
- Author:
Cai-juan ZHANG
1
;
Xiao-yan JIN
1
;
Min-yi QIU
1
;
Chi ZHANG
1
;
Ling-long LUO
1
;
Min WANG
2
;
Bao-sheng ZHAO
2
;
Xue-yong WANG
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
2. Beijing Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Research Article
- Keywords:
Ziya Biti tablet;
type 2 diabetic(T2DM);
diabetes;
LC-MS
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(8):141-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the hypoglycemic effect of Uygur medicine Ziya Biti tablet on the type 2 diabetic rats, and analyze the hypoglycemic mechanism based on metabolomic techniques. Method: According to the results of clinical research about different origins of Ziya Biti tablet, the optimal composition was screened out; type 2 diabetic rats were taken as an experimental object in the pharmacodynamic experiments;the control group and model group were given the same dose of normal saline, Ziya Biti bablet group was given 300 mg·kg-1, the metformin group was given 300 mg·kg-1 metformin hydrochloride. The fasting blood and weight changes of the experimental group after the treatment were recorded and compared with normal group; ultra-high performance liquid chromatography-linear ion trap/electrostatic field orbit trap combined-type high resolution mass spectrometry (UHPLC-LTQ/Orbitrap MS) technology was used to conduct the metabolomics analysis on the rat serum, and principal component analysis (PCA), partial least squares discriminant analysis (OPLS-DA) on different groups of rat serum metabolites were performed to identify potential biomarkers. Result:Compared with the model group, the rats in the Uygur medicine Ziya Biti tablet showed a healthy states, and the blood glucose were decreased(P<0.05), which indicated that Uygur medicine Ziya Biti tablet had a certain hypoglycemic effect on type 2 diabetic rats. Eight biomarkers were finally obtained according to the results. Compared with model group, L-valine and propionylcarnitine in the Uygur medicine group were decreased(P<0.05), while sphingosine-1-phosphate, LPC (16:1/0:0), LPC (18:0/0:0), LPC (18:2/0:0), LPC 20:1/0:0), PC (19:0/0:0) were elevated(P<0.05). Conclusion:The experimental results showed that Uygur medicine Ziya Biti tablet can reduce the blood glucose of type 2 diabetic rats and allivate general physiological characteristics. The mechanism of action may be related to the improvement of amino acid metabolism and lipid metabolism.
