Simultaneous Determination of Seven Constituents in Qinggan Lidan Mixture by HPLC
10.13422/j.cnki.syfjx.20190817
- VernacularTitle: HPLC同时测定清肝利胆口服液中7种成分
- Author:
Yu-xia LOU
1
;
Xiang-yang LI
2
;
Zhen-guo LI
2
Author Information
1. Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China
2. Henan Institute for Food and Drug Control, Zhengzhou 450018, China
- Publication Type:Research Article
- Keywords:
Qinggan Lidan mixture;
geniposide;
chlorogenic acid;
neochlorogenic acid;
cryptochlorogenic acid;
isochlorogenic acid A;
isochlorogenic acid B;
isochlorogenic acid C
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(8):128-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for simultaneous determination of geniposide,chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A,B and C in Qinggan Lidan mixture,in order to provide references for its quality control. Method: The analysis of methanol extract of this drug was performed on a 35℃ Luna C18 column (4.6 mm×250 mm,5μm),with the mobile phase comprised of acetonitrile-0.4% phosphoric acid flowing at 1.0 mL·min-1 in a gradient elution mode (0-10 min,8%-12%A;10-30 min,12%A;30-60 min,12%-35%A),and the detection wavelengths were set at 238 and 327 nm. Result:Geniposide,chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A,B and C were completely separated,and well separated from other constituents. The linear ranges of geniposide,chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A,B and C were 0.188-2.355,0.083-1.040,0.074-0.920,0.075-0.940,0.064-0.800,0.076-0.955,0.071-0.888 μg (r ≥ 0.999 0),respectively. The average recoveries were 99.45%,98.45%,99.06%,98.50%,98.16%,101.01%,96.93%,with the RSDs of 0.5%,1.8%,1.3%,2.4%,2.3%,1.6%,1.6%,respectively.The contents of geniposide,chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A,B and C were 3.420-3.794,0.835-0.890,1.222-1.275,1.064-1.210,0.377-0.398,0.419-0.464 and 0.362-0.405 g·L-1,respectively. Conclusion:This method can be used for simultaneous determination of muti-ingredients in Qinggan Lidan mixture,and the established method is simple and accurate,with a good reproducibility and high sensitivity. It can be used for the quality control of Qinggan Lidan mixture.
