Callus Induction from Amomum villosum
10.13422/j.cnki.syfjx.201907819
- VernacularTitle: 阳春砂愈伤组织的诱导
- Author:
Ming-xiao LI
1
;
Shu-hui CHEN
1
;
Jing SU
2
;
Li-yun TANG
3
;
Jie XU
1
;
Zhuo-hang HE
1
;
Guo-zhen HE
1
Author Information
1. College of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
2. Yangchun Field Test and Demonstration of Amomum villosum, Yangchun 529600, China
3. College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
- Publication Type:Research Article
- Keywords:
Amomum villosum;
tissue culture;
rhizome bud;
stem segment;
root tip segment
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(8):114-119
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To set up a callus induction system for Amomum villosum by tissue culture. Method: The rhizome buds of A. villosum and stem segments,root tip segments of sterile A. villosum plantles were used as explants and cultured in MS media with different concentrations of 6-BA,NAA and 2,4-D (the pH of each medi is about 5.8). A callus induction system was established to explore the effect of different explants and different medium on callus induction for A. villosum. Result:The findings showed that the rhizome buds and sterile plantlet stems and root tip segments of three different explants can be successfully induced into calli. The most suitable medium for callus induction from rhizome buds and sterile plantlet stems was MS with 6-BA (1.5 mg·L-1),2,4-D (1.0 mg·L-1) and NAA (0.5 mg·L-1) with the highest induction rates of 15% and 60% respectively. MS medium combined with 6-BA (2.0 mg·L-1),2,4-D (1.0 mg·L-1) and NAA (1.0 mg·L-1) was the most suitable proposal for inducing the callus from sterile root tip segments with the highest induction rate of 76%. Conclusion:Under certain culture conditions,rhizome buds,stem or root tip segments of sterile plantlet can be effectively induced into callus. The callus induction system of A. villosum is preliminarily established, and root tip segments of sterile plantlet are the optimal explant.
