Analysis of Bacterial Flora Structure of Patients with Cerebral Hemorrhage Due to Hyperactivity of Liver-Yang by 16S rRNA Gene Sequencing Technique
10.13422/j.cnki.syfjx.20190550
- VernacularTitle: 16S rRNA基因测序技术分析肝阳上亢型脑出血患者的肠道菌群结构
- Author:
Yu-si LI
1
;
Hua-chong XU
2
;
Jun-yue WANG
3
;
Fan-chao ZHENG
1
;
Li DENG
2
;
Jing-bo LI
1
;
Long-jiao CHEN
1
;
Huai-tao YUAN
4
;
Xiao-yin CHEN
2
Author Information
1. School of Basic Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510000, China
2. School of Traditional Chinese Medicine, Jinan University, Guangzhou 510000, China
3. The Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510000, China
4. The first Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510000, China
- Publication Type:Research Article
- Keywords:
16S rRNA gene sequencing technology;
hyperactivity of liver-Yang;
acute cerebral hemorrhage;
flora structure;
intestinal flora;
Venn analysis;
species level
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(8):83-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the difference of intestinal flora between the patients with acute cerebral hemorrhage due to hyperactivity of liver-Yang and the healthy population. Method: The fecal samples of 9 patients with acute cerebral hemorrhage due to hyperactivity of liver-Yang from the first affiliated hospital of Guangzhou university of traditional Chinese medicine in 2018 were selected as observation group,and 6 stool samples from healthy subjects were selected as the control group.The total bacterial DNA was extracted from the two groups of samples,amplified according to the 16S rRNA V4 region,and paired-end sequencing was performed on the Illumina MiSeq platform.The sequencing results were analyzed by bioinformatics analysis software.The flora composition and structure of the samples from two groups were compared. Result:Venn analysis of operational taxonomic units(OTU) showed significant difference in OTU numbers between the observation group and control group.Partial least squares-discriminant analysis(PLS-DA) showed that there was a significant difference in the composition of intestinal flora between patients with acute cerebral hemorrhage and healthy subjects.On the analysis of species and abundance,at the classification level of phylum,compared with the control group,the ratio of relative abundance values of Firmicutes and Bacteroidetes(F/B) in the observation group was significantly increased,and the relative abundance of Verrucomicrobia was significantly decreased(P<0.05);at the classification level of genus,there were significant differences between the two groups in the Prevotella,Bacteroides,Akkermansia,Blautia and Acidaminococcus(P<0.05,P<0.01),the ratio of relative abundance values of Bacteroides and Prevotella(B/P) in the observation group was significantly higher than that of the control group;at the classification level of species,there were significant differences between the two groups in P. copri,A. muciniphila,B. ovatus,B. fragilis and Ruminococcus callidus(P<0.05,P<0.01).Principal coordinate analysis showed that the two groups of samples were significantly separated,indicating that there were significant differences in flora structure between the two groups. Conclusion:Acute cerebral hemorrhage due to hyperactivity of liver-Yang is associated with structural disorder of intestinal flora,which is closely related to the decrease in relative abundance of P. copri and A. muciniphila.
