Accumulation and Toxicity of Mercury in Zhuhong Ointment in Rats with Damaged Skin Model
10.13422/j.cnki.syfjx.20190823
- VernacularTitle: 朱红膏中汞在破损皮肤模型大鼠体内的蓄积及毒性
- Author:
Yue TIAN
1
;
Jian-mei HUANG
1
;
Xiao-ye WANG
1
;
Yan-ling LIU
1
;
Cong ZHANG
1
;
Zheng-kun LI
1
;
Qiang WANG
1
;
Rui-chao LIN
1
Author Information
1. School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China
- Publication Type:Research Article
- Keywords:
Zhuhong ointment;
mercury;
skin damage;
toxicity
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(8):34-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the accumulation of mercury in liver, kidney and brain of rats and its toxicity on liver and kidney after 4 weeks of administration of different doses of Zhuhong ointment, in order to provide data reference for the safe clinical use of Zhuhong ointment. Method: Forty-four Sprague-Dawley (SD) rats were randomly divided into 4 groups:control group, normal-dose group (1.875 mg·kg-1), medium-dose group (37.5 mg·kg-1), and high-dose group (75 mg·kg-1). After transdermal administration for consecutive 4 weeks, the mercury content in the urine, blood, liver, kidney and brain of the rats was measured. In addition, serum alanine aminotransferase (ALT), serum aspartate aminotransferase (AST), serum urea nitrogen (BUN), and serum creatinine (SCr), urine β2-miSCroglobulin (β2-BMG) and urine N-acetyl-beta-D glucosidase (NAG) contents were measured, and pathological morphology changes of liver and kidney were observed. Result:Compared with the control group, the levels of blood mercury and urine mercury in Zhuhong ointment groups showed significant increases after administration for 4 weeks(P<0.05,P<0.01). The level of serum ALT was significantly elevated in the high-dose group, and serum SCr was significantly elevated in middle and high-dose groups (P<0.05,P<0.01). Biochemical index in the normal dose remained unchanged. Medium and high doses of Zhuhong ointment destroyed the structure of the hepatic lobule and the radial arrangement of the liver monolayer, shrank the lumen and glomerular, and even led to glomerular balloon enlargement(P<0.05,P<0.01). Normal dose of Zhuhong ointment had no significant effect on liver tissue morphology, but slightly changed kidney tissue morphology. Conclusion:Zhuhong ointment is not toxic at the normal dose, but long-term use can lead to the accumulation of mercury in liver, kidney and bra, which causes liver and kidney toxicity. This study did not find a more sensitive indicator of liver and kidney toxicity than liver and kidney pathology. However, because the rising levels of urinary mercury and blood mercury may predict toxicity, the relationship between mercury exposure and toxicity could be further studied. This study provides a reference for the rational use and toxicity monitoring of Zhuhong ointment.
