Chemical Components in Pinelliae Rhizoma by UPLC-Q-TOF-MS/MS
10.13422/j.cnki.syfjx.20190107
- VernacularTitle: 采用UPLC-Q-TOF-MS/MS分析半夏药材中的化学成分
- Author:
Xing-ying ZHAI
1
;
Ling ZHANG
1
;
Bing-tao LI
1
;
Yu-lin FENG
1
;
Guo-liang XU
1
;
Shi-lin YANG
1
;
Chen JIN
1
Author Information
1. Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
UPLC-Q-TOF-MS;
Pinelliae Rhizoma;
chemical components;
toxicity
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(7):173-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify the chemical constituents of Pinelliae Rhizoma(BX)by ultrahigh-pressure liquid chromatography coupled with electrospray time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS). Method: Chromatograpic separation was performed on a shim-pack xR-ODS Ⅲ column (2.1 mm×75 mm,1.6 μm) using a gradient elution program with mixtures of acetonitrile and 0.1% formic acid-water as mobile phases at a flow rate of 0.25 mL·min-1. UPLC-Q-TOF-MS/MS was developed for rapid and high-throughput screening of the preliminary chemical profile of BX in positive ion modes. Result: Ninety chemical components were found in BX,according to the accurate Charge-mass Ratio and the MS/MS data,retention time of reference standard,references or databases,the fragmentation regularities of mass spectra. Eighty were identified preliminarily,including 7 alkaloids,8 poly-alcohols,12 fatty glycerides,5 flavonoids,12 lysophosphatidylcholines (LPCs),10 alcohol amines,11 amino acids,11 amides and 4 other type. Conclusion: LPCs were first found in BX. Because BX has certain toxicity,it is found that BX contains LPCs by analyzing the chemical constituents of BX,which can cause inflammatory reaction and neuronal myelin sheath loss and degeneration. Therefore,the analysis of the chemical composition of BX can explain the causes of the toxicity and provide a foundation for the basic research and quality control of the potency of BX.
