Analysis of Solubilization of Fatty Oil from Euphorbiae Semen on Four Euphorbia Factors Based on Thinking of Self-assembled Micelles in vivo
10.13422/j.cnki.syfjx.20182302
- VernacularTitle: 基于体内自组装胶束思维分析千金子脂肪油对4种千金子素的增溶作用
- Author:
Tong SU
1
;
Rui YUAN
1
;
Chao ZHANG
1
;
Hui-hui GAO
1
;
Ying-zi WANG
2
;
Xiu-mei SUN
1
Author Information
1. School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China
2. Beijing University of Chinese Medicine, Beijing 100102, China
- Publication Type:Research Article
- Keywords:
Euphorbiae Semen;
fatty oil;
bile salt;
mixed micelles;
solubilization;
diterpene esters;
Euphorbia factors
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(7):160-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The solubilization of 4 compounds(Euphorbia factor L1,L2,L3 and L8),caused by mixed micelles self-assembled from fatty oil of Euphorbiae Semen and bile salt of intestinal juice,was researched in the simulated human intestinal environment. Method: The mixed micelles were prepared with different amounts of fatty oil of Euphorbiae Semen.The transmission electron microscope(TEM) was used to observe the morphology of the micelles.Particle size detector was used to determine the particle size and Zeta potential.HPLC was used to assay the solubility of these 4 compounds.The variation tendency of the total dissolution of these 4 compounds with the change of standing time was observed. Result: Particle size of the mixed micelles was uniform and its morphology was spherical.The absolute values of Zeta potential were less than 20 mV.When the amount of sodium deoxycholate was fixed 4.96 g·L-1,the solubility of these 4 compounds with the concentration of fatty oil at 0.1-4 g·L-1 were significantly greater than that at the dosage of 0 g·L-1.The solubility of these 4 compounds in the micelles formed by fatty oil was 1.3 to 4 times as much as the micelles without fatty oil.The micelles was stable for 36 h. Conclusion: The micelles self-assembled from fatty oil of Euphorbiae Semen and bile salt of intestinal juice,have significant solubilization effect on Euphorbia factor L1,L2,L3 and L8.This research can lay the foundation for clarifying the detoxification mechanism of removing fatty oil and making frostlike powder from the perspective of pharmaceutics.
