Preparation of Oxymatrine Phospholipid Complex Solid Lipid Nanoparticles Lyophilized Powder and Evaluation of Its Quality
10.13422/j.cnki.syfjx.20190306
- VernacularTitle: 氧化苦参碱磷脂复合物固体脂质纳米粒冻干粉的制备与质量评价
- Author:
Jun-li YAN
1
;
Wan-rong LI
1
;
Jia-jia YANG
1
;
Yi WANG
1
;
Zhi-yong HE
1
;
Feng JIANG
1
;
Xue ZHOU
1
;
Lin-jing WU
1
;
Shi-quan GAN
1
;
Heng WANG
2
;
Xiang-chun SHEN
1
;
Ling TAO
1
Author Information
1. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Engineering Center of Efficient Utilization of Natural Medicine Resources, High Educational Key Laboratory of Natural Medicinal Pharmacology and Druggability of Guizhou Province, Key Laboratory of Optimal Utilization of Natural Medicinal Resources, Guizhou Medical University, Guiyang 550025, China
2. Qiannan Medical College for Nationalities, Duyun 558003, China
- Publication Type:Research Article
- Keywords:
oxymatrine phospholipid complex;
pseudo-ternary phase diagram;
solid lipid nanoparticles;
lyophilized powder;
in vitro release performance;
encapsulation efficiency;
lyoprotectant
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(7):146-152
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare oxymatrine phospholipid complex solid lipid nanoparticles(OMT-PC-SLN) lyophilized powder and evaluate its pharmaceutical properties. Method: Pseudo-ternary phase diagram was employed to optimize the formula of microemulsion;single factor experiments were adopted to optimize the formulation process of OMT-PC-SLN lyophilized powder with encapsulation efficiency as index;the morphology of this preparation was observed by transmission electron microscope(TEM).The particle size was measured by particle size analyzer and the in vitro release performance of OMT-PC-SLN lyophilized powder was examined. Result: Optimal formulation process was as following:taking soybean phospholipid and polyethylene glycol 15-hydroxystearate(Kolliphor HS 15) as the emulsifier,ethanol as co-emulsifier,ratio of emulsifier to co-emulsifier(Km)=3:2,oil phase:(emulsifier+co-emulsifier)=1:9,oxymatrine phospholipid complex-stearic acid-soybean phospholipid-Kolliphor HS 15-ethanol(30:100:180:360:360);taking 50 mL of 4%mannitol solution as the external aqueous phase,ice bath stirring at 1 000 r·min-1 and solidifying for 1 h,precooled at -20℃ for 24 h,took out and dried for 24 h.OMT-PC-SLN lyophilized powder was spherical in appearance with encapsulation efficiency of (38.09±1.24)%,average particle size of 785.5 nm,polydispersity coefficient(PDI) of 0.456 and the Zeta potential of -24.82 mV.The cumulative release rates of OMT-PC-SLN lyophilized powder were 72.63%at 2 h and 98.42%at 12 h;the cumulative release rate of oxymatrine(crude drug) was 98.60%at 2 h. Conclusion: This optimized formulation process of OMT-PC-SLN lyophilized powder is stable with good repeatability;compared with oxymatrine,OMT-PC-SLN lyophilized powder has a certain sustained-release effect.
