Effect of Capsaicin on Proliferation and HMGB1 and IL-6 Expressions in Hepatoma SMMC-7721 Cells
10.13422/j.cnki.syfjx.20190721
- VernacularTitle: 辣椒碱对肝癌SMMC-7721细胞增殖及HMGB1,IL-6表达的影响
- Author:
Chan-chan XIAO
1
;
Mao-jian CHEN
1
;
Fan-biao MEI
1
;
Guan-hua REN
1
;
Tian-ren HUANG
1
;
Ji-lin LI
1
;
Wei DENG
1
Author Information
1. Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China
- Publication Type:Research Article
- Keywords:
capsaicin;
SMMC-7721 cells;
proliferation;
high mobility group box 1 (HMGB1);
interleukin-6(IL-6)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(7):89-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of capsaicin on proliferation in human hepatoma SMMC-7721 cells and its possible molecular mechanism. Method: Capsaicin (50,100,150,200,250,300 μmol·L-1) groups and blank group were set up. The cell viability was detected by cell counting kit-8 (CCK-8) assay after SMMC-7721 cells were treated with capsaicin (50,100,150,200,250,300 μmol·L-1) for 24, 48, 72 h. The morphological changes were observed under an inverted microscope after SMMC-7721 cells were treated with capsaicin (150,200,250 μmol·L-1) for 24 h. The mRNA expression levels of high mobility group box 1 (HMGB1) and interleukin-6(IL-6) were measured by Real-time PCR after SMMC-7721 cells were treated with capsaicin (150,200,250 μmol·L-1) for 24 h. The levels of HMGB1 and IL-6 in cell culture supernatant were detected by enzyme-linked immunosorbent assay(ELISA) after SMMC-7721 cells were treated with capsaicin (150,200,250 μmol·L-1) for 24 h. Result: Compared with the blank group, there was no significant difference between 50 and 100 μmol·L-1 capsaicin groups treated for 24, 48, 72 h; after treated with the other concentrations of capsaicin (150, 200, 250, 300 μmo·L-1) at different time points, the proliferation inhibition rate was statistically significant (P<0.05), with significant concentration and time-dependent effect; compared with the blank group, capsaicin (150, 200, 250 μmol·L-1) groups showed different degrees of morphological changes in SMMC-7721 cells, which became round and wrinkled, with a poor attachment and more exfoliation; compared with the blank group, the mRNA expressions of HMGB1 and IL-6 in SMMC-7721 cells of capsaicin (150, 200, 250 μmol·L-1) groups were significantly down-regulated (P<0.05), and the secretions of HMGB1 and IL-6 were significantly decreased in the supernatant (P<0.05). Conclusion: Capsaicin inhibits cell proliferation of SMMC-7721 cells, and the possible mechanism may be related to the down-regulation of HMGB1 and IL-6 at the mRNA and protein levels.
