Inhibitory Effect of Capsaicin on Growth of Breast Cancer MDA-MB-231 Cells Transplanted Tumor in Nude Mice
10.13422/j.cnki.syfjx.20190723
- VernacularTitle: 辣椒碱对乳腺癌MDA-MB-231细胞裸鼠移植瘤生长的抑制作用
- Author:
Mao-jian CHEN
1
;
Wei JIANG
1
;
An-yun MAO
1
;
Qing-hong QIN
1
;
Qi-xing TAN
1
;
Chan-chan XIAO
1
;
Zhi-jie LIANG
1
;
Wei-ping YANG
1
;
Chang-yuan WEI
1
Author Information
1. Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China
- Publication Type:Research Article
- Keywords:
capsaicin;
breast cancer;
transplanted tumor in nude mice;
high mobility group box 1 (HMGB1);
Toll-like receptor 4(TLR4)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(7):82-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the inhibitory effect of capsaicin on the growth of breast cancer MDA-MB-231 cells transplanted tumour in nude mice and its possible molecular mechanism. Method: Transplanted tumor model of breast cancer MDA-MB-231 cells in nude mice were established. Then the tumor-bearing mice were randomly divided into 4 groups:model group, and low, medium and high-dose capsaicin groups (5, 10, 20 mg·kg-1). Mice of low, medium and high-dose capsaicin groups (5, 10, 20 mg·kg-1) were intraperitoneally injected with the corresponding dose of capsaicin, and the model group was injected with the same volume of phosphate buffer saline (PBS), once every 3 days, for a total of 8 times in succession. Body weight of mice and transplantation tumor volume were measured before each injection of capsaicin. Mice of each group were put to death 24 h after the last administration, and then the tumor volume, mass and the tumor inhibitory rate were calculated. The protein expression levels of high mobility group box 1 (HMGB1) and Toll-like receptors 4(TLR4) were measured by immunohistochemistry and Western blot. Result: No significant difference was observed between each group in body weight. However, compared with the model group, capsaicin (5, 10, 20 mg·kg-1) remarkably inhibited the tumor volume and mass (P<0.05,P<0.01), and increased the tumor inhibitory rate (P<0.01) in a dose-dependent manner. Capsaicin (5, 10, 20 mg·kg-1) also markedly inhibited the protein expression levels of HMGB1 and TLR4 (P<0.01). Conclusion: Capsaicin remarkably inhibits the growth of breast cancer MDA-MB-231 cells transplanted tumour in nude mice, and the possible mechanism may be related to the down-regulation of HMGB1 and TLR4 at the protein level.
