Variation Pattern Analysis of Momordin Ⅰc Content and High-quality Germplasm Screening in Kochiae Fructus from Various Origins
10.13422/j.cnki.syfjx.20190611
- VernacularTitle: 地肤子中地肤子皂苷Ⅰc含量变异规律与优良种质筛选
- Author:
Xiao-yan WU
1
;
Li-hong FANG
2
;
Wei-qi LYU
3
;
Xiao-fei YING
1
Author Information
1. Lishui Central Hospital, Lishui 323000, China
2. Lishui Maternal and Child Health Hospital, Lishui 323000, China
3. Lishui People's Hospital, Lishui 323000, China
- Publication Type:Research Article
- Keywords:
Kochiae Fructus;
momordin Ⅰc;
variation pattern;
high-quality germplasm
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(6):183-188
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To provide evidence and lay a foundation for quality control and resources utilization of Kochiae Fructus. Method:A total of 23 samples of Kochiae Fructus were collected from the main distribution areas in China. Momordin Ⅰc content was determined via HPLC-ELSD method, and its correlation with growth traits and habitat factors was analyzed using SPSS 23.0 software,as well as the principle component and clustering analysis. Result:Momordin Ⅰc content had a benign linear relationship with HPLC-ELSD peak area within the range between 29.8 mg·L-1 and 178.8 mg·L-1 (R2=0.999 8),with the quantitation limit at 5.79 mg·L-1 and the detection limit at 2.36 mg·L-1. Significant differences (P<0.01) were found in momordin Ⅰc contents among 23 Kochiae Fructus germplasms. According to momordin Ⅰc content,all samples tested met the requirements in the Chinese Pharmacopeia published in 2015.Origin,growth traits,and habitat factors could impact momordin Ⅰc content accumulation in Kochiae Fructus. Moreover,the content had a negative correlation with plant height,stem diameter,leaf length,leaf width,fruit diameter and longitude,and a positive correlation with ratio of length to width of leaves,altitude and latitude. Those from Shanxi,Jilin,and Shandong provinces had a higher momordin Ⅰc content. The characters tested could be simplified to four principle components, and their cumulative contribution rate was 95.831%. Kochiae Fructus samples tested could be divided into four groups,and those from group Ⅰ had superior growth traits, and those from group Ⅲ had higher momordin Ⅰc content. Conclusion:The optimized HPLC-ELSD method for determining momordin Ⅰc content of Kochiae Fructus is convenient,accurate and reproducible,and could be used to control the herbs quality. Those cultivated in a lower longitude,higher altitude,and higher latitude region could accumulate more momordin Ⅰc content. More attention shall be paid to germplasm in Shanxi and Zhejiang with superior resources screening of Kochiae Fructus.
