Analysis of Effective Substance of Qingre Chushi Decoction by UPLC-Q-TOF-MS
10.13422/j.cnki.syfjx.20190614
- VernacularTitle: UPLC-Q-TOF-MS辨析清热除湿汤药效物质基础
- Author:
Xu-yang HAN
1
;
Lu ZHANG
1
;
Bing PENG
1
;
Hong WANG
1
;
Ping LI
1
;
Zu-ping ZENG
1
Author Information
1. Beijing Key Laboratory of Clinic and Basic Research with Traditional Chinese Medicine(TCM) on Psoriasis, Beijing Institute of TCM, Beijing Hospital of TCM, Capital Medical University, Beijing 100010, China
- Publication Type:Research Article
- Keywords:
UPLC-Q-TOF-MS;
Qingre Chushi decoction;
principal component analysis;
orthogonal partial least squares-discriminant analysis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(6):150-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To characterize and compare the chemical information of four extracts of Qingre Chushi (QRCS) decoction by liquid chromatography-mass spectrometry (LC-MS), and combine the chemical information of the four extracts with their results of anti-inflammatory effect for a multivariate statistical analysis, in order to identify the compounds directly relating to the anti-inflammatory effects of QRCS decoction. Method:Four extracts of QRCS decoction were characterized by UPLC-Q-TOF-MS:①ethanol extract+water extract,② ethanol extract+supernatant after water extraction and alcohol precipitation, ③ ethanol extract+precipitation after water extraction and alcohol precipitation,and ④ standard decoction. On the basis of the results of inhibition of the four above extracts on xylene-induced ear swelling in mice,multivariate statistical analysis[principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA)] were carried out to lock the chromatographic peaks with significant differences between group ① (the best pharmacological action group) and group ④ (standard decoction group). According to the accuracy of quasi-molecular ion and fragment ion data,and the reference materials and literature data,those chromatographic peaks were identified. Result:PCA could cluster the four extracts of QRCS decoction,and the differences between groups was reflected in the distance between groups. Group ④ (standard decoction) had the most significant differences with the other three groups, especially in the first principal component; group ① (ethanol extract+water extract),group ② (ethanol extract+supernatant after water extraction and ethanol precipitation) and group ③ (ethanol extract+precipitation after water extraction and ethanol precipitation) had certain differences in the second principal component. OPLS-DA was used to compare group ① (the best pharmacological action group) and group ④ (standard decoction group). Eleven chromatographic peaks with great contribution and high reliability to group differences,were identified as gentiopicrin,skimmin,baicalin,baicalin isomer,wogonoside,5,6,7-trihydroxy-8-methoxyflavone-7-O-glucurodonaldehyde,5,6-dihydroxy-6,8,2',3'-tetramethoxyflavone,salicin-6-C-arabinose-8-C-glucoside,plantamajoside and glycyrrhizic acid. Conclusion:In the mode of pectrum-effect combination, this study explores and identifies compounds relating to the anti-inflammatory effect of QRCS decoction,so as to provide the basis for screening the extraction and purification process and optimizing the formulation of preparation of Qingre Chushi decoction.