Analysis of Fingerprint of Standard Decoction of Citri Reticulatae Pericarpium Based on Spectroscopic Method
10.13422/j.cnki.syfjx.20182407
- VernacularTitle: 基于光谱法的陈皮饮片标准汤剂指纹图谱分析
- Author:
Tao LIU
1
;
Yu-xue ZHANG
1
;
Jia ZHANG
1
;
Meng-die XIE
1
;
Chun-hui PAN
2
;
Hui YANG
1
;
Yan-jun DENG
1
;
Tian-yu JIANG
1
;
Yu-ling XU
1
Author Information
1. College of Pharmacy and Biological Engineering, Chengdu University, Chengdu 610106, China
2. Sichuan Decheng Animal Health Products Co. Ltd., Deyang 618100, China
- Publication Type:Research Article
- Keywords:
Citri Reticulatae Pericarpium;
standard decoction;
infrared fingerprint;
ultraviolet fingerprint;
double index sequence analysis method;
hesperidin;
dry extract rate
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(6):136-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the fingerprint of standard decoction of Citri Reticulatae Pericarpium and evaluate its quality. Method:According to the preparation conditions of the standard decoction,15 batches of standard decoction of Citri Reticulatae Pericarpium were prepared.HPLC was employed to determine the content of hesperidin in this standard decoction.Ultraviolet spectroscopy(UV) and infrared spectroscopy(IR) were used to establish the fingerprint of standard decoction of Citri Reticulatae Pericarpium.The correlation coefficient method and double index sequence analysis method were used to compare and analyze the spectra of different batches of this standard decoction. Result:The content of hesperidin in 15 batches of this standard decoction were 0.82%-2.60%,and the measured value of dry extract rate was 32.02%-46.11%.Compared with ultraviolet and infrared control fingerprint,the fingerprint similarities of the standard decoction of each batch were > 0.897 and > 0.942,respectively.The double index analysis results showed that the common peak ratio was more than 62.50%,variation peak ratio was less than 46.67%. Conclusion:The quality evaluation method established in this study can be used for systematic evaluation of standard decoction of Citri Reticulatae Pericarpium,and it can provide theoretical reference for the formulation of quality standard of Citri Reticulatae Pericarpium dispensing granules and other related preparations.
