Optimization of Purification Process of Total Alkaloid Extract of Berberis dictyophylla Cortex by Macroporous Resin and Investigation of Its Quality Standard
10.13422/j.cnki.syfjx.20190555
- VernacularTitle: 小檗皮总生物碱提取物的大孔树脂纯化工艺与质量标准考察
- Author:
Hui FENG
1
;
Ya ZHAO
1
;
Xiao-yan WANG
1
;
Yu-nan XIANG
1
;
Lu HAO
1
;
Qi-en LI
2
;
Xian-jia LI
2
;
Xian-rong LAI
1
Author Information
1. Ethnic Medicine School, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
2. Tibetan Medical College, Qinghai University, Xining 810000, China
- Publication Type:Research Article
- Keywords:
Berberis dictyophylla cortex;
total alkaloids;
macroporous resin;
purification process;
quality standard;
characteristic chromatogram;
berberine hydrochloride
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(16):97-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize purification process of total alkaloid extract of Berberis dictyophylla cortex by macroporous resin,and to establish its quality standard. Method: Acid dye colorimetry was used to investigate the purification process of total alkaloid extract of B. dictyophylla cortex,the process parameters included concentration of sample solution,speed of sampling,diameter-height ratio of resin column,water washing amount,concentration and dosage of eluent,flow rate of elution,etc.In order to determine the optimum process,HPLC was employed to determine the contents of four alkaloids(magnoflorine,jatrorrhizine hydrochloride,palmatine hydrochloride,and berberine hydrochloride) with mobile phase of acetonitrile-0.1% phosphoric acid aqueous solution for gradient elution and detection wavelength at 270 nm.After being purified,quality standard of total alkaloid extract of B. dictyophylla cortex was investigated according to the requirements in the 2015 edition of Chinese Pharmacopoeia. Result: Optimal purification conditions were as following:10 g of HPD100 macroporous adsorption resin with a column diameter-height ratio of 1:8,sampling solution concentration of 11 g·L-1,the loading flow rate of 1 mL·min-1,sampling solution volume of 50 mL,washed with 4 BV of water(1 BV=15 mL) and added 9 BV of 30% ethanol,after being purified,the transfer rate of total alkaloids was>80%,and its purity was>65%.The quality standard of total alkaloid extract of B. dictyophylla cortex was established,there were 19 common peaks in the characteristic chromatogram,and the overall similarity was>0.99. Conclusion: This optimized purification process is stable and feasible, and the established quality standard is controllable.
