Correlation Between RhoA Gene in Wnt/PCP Signaling Pathway and Traditional Chinese Medicine Syndrome of AECOPD

Jun Zhang; Zheng-xing GE; Xun Zhou; Yi Yang

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Correlation Between RhoA Gene in Wnt/PCP Signaling Pathway and Traditional Chinese Medicine Syndrome of AECOPD

VernacularTitle: Wnt/PCP信号通路中RhoA mRNA与AECOPD中医证型的相关性
Author: Jun ZHANG ¹ ; Zheng-xing GE ¹ ; Xun ZHOU ¹ ; Yi YANG ¹
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1. The 2^th Hospital Affiliated to Guiyang University of Chinese Medicine, Guiyang 550003, China
Publication Type:Research Article
Keywords: Ras homolog gene family member A (RhoA) gene; acute exacerbation chronic obstructive pulmonary disease(AECOPD); traditional Chinese medicine(TCM) syndrome type
From: Chinese Journal of Experimental Traditional Medical Formulae 2019;25(16):73-77
CountryChina
Language:Chinese
Abstract: Objective: To investigate the correlation between Ras homolog gene family member A (RhoA) gene in Wnt/PCP signaling pathway and acute exacerbation chronic obstructive pulmonary disease (AECOPD) traditional Chinese medicine(TCM)syndrome, attempting to provide an objective standard for the diagnosis of AECOPD TCM syndrome. Method: The 100 AECOPD patients were collected and divided into 5 groups:phlegm turbid obstructing lung syndrome,, phlegm-heat obstructing lung syndrome, syndrome of orifices confused by phlegm, deficiency of pulmonary and renal Qi, and edema due to yang deficiency, with 20 people in each group. 15 normal people were selected as a normal control group. All patients received fasting hemospasia, using a kit to extract blood total RibonucleicA(RNA) according to instructions. Real-time quantitative polymerase chain reaction (Real-time PCR) was adopted to detect the mRNA expression of RhoA gene in blood of patients with AECOPD TCM syndrome, and to explore the correlation. Result: There was no difference between phlegm-heat obstructing lung syndrome group and syndrome of orifices confused by phlegm group. The mRNA expression of RhoA gene in phlegm turbid obstructing lung syndrome group, phlegm-heat obstructing lung syndrome group, syndrome of orifices confused by phlegm group, deficiency of pulmonary and renal Qi group, and edema due to Yang deficiency group were significantly higher than that in normal group (P<0.01). The mRNA expression of RhoA gene in five TCM syndrome groups was as follows:phlegm turbid obstructing lung syndrome < phlegm-heat obstructing lung syndrome/syndrome of orifices confused by phlegm < deficiency of pulmonary renal Qi < edema due to Yang deficiency. There was a positive linear correlation between the relative expression of RhoA mRNA and above five AECOPD TCM syndrome groups. Conclusion: The significant difference in mRNA relative expression of RhoA gene in Wnt/PCP signaling pathway among the five AECOPD TCM syndrome groups may provide some objective diagnostic criteria for AECOPD TCM syndromes and reveal their disease severity.