Some results of malaria parasite species collected from Daknong province and analysis of drug resistance in P.Palciparum by the polymerase chain reaction.
- Author:
Nhan Hanh Doan
;
Duong Van Nguyen
;
Thanh Viet Ngo
;
Toan Quoc Tran
;
Phuong Nhu Vo
- Publication Type:Journal Article
- Keywords:
Malaria parasite species;
polymerase chain reaction;
P.Palciparum;
drug resistance
- MeSH:
Malaria parasite species;
polymerase chain reaction;
P.Palciparum;
drug resistance
- From:Journal of Malaria and parasite diseases Control
2003;0(1):25-30
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: In many years, National Institute of Malariology, Parasitology and Entomology conducted collection, storage and preservation of malaria parasites species \r\n', u'Objective: to evaluate some results of malaria parasite species collected from Daknong province and analysis of drug resistance in P.Palciparum by the polymerase chain reaction.\r\n', u'Subject and method: Malaria parasite species collected from Daknong province in 2006. Thirty-five isolates were confirmed to be resistant with chloroquin by in vitro test. Polymerase chain reaction-restriction fragment leng polymorphism were used. \r\n', u'Results: 55 Plasmodium jalciparum. 7 Plasmodium vivax. 4 Plasmodium malariae. 1 Plasmodium ovale samples were collected from the malaria patients. A preliminary analysis of drug-resistant mutations in the Plasmodium jalciparum chloroquine resistance transpory (pfcrt) and P Jalciparummulti-drug resistant genes showed that the change of the order of amino-acid of Plasmodium jalciparum was closely correlated to chloroquine resistance in 35 isolates at the mutant allele 76 of pfcrt gene of chloroquine resistant Plasmodiuntjalciparum isolates. \r\n', u'Conclusion: These results contributed to supplement malaria parasite species that were stored in National Institute of Malariology, Parasitology ad Entomology.\r\n', u'
