Study on Protective Effect of Huayu Qutan Decoction on Vascular Dementia
10.13422/j.cnki.syfjx.20191539
- VernacularTitle: 化瘀祛痰方对血管性痴呆的保护作用机制探讨
- Author:
Dong-yu MIN
1
;
Zhe ZHANG
1
;
Shu ZHU
2
;
Le GUAN
1
;
Ye-tao JU
1
;
Dong-ni WANG
1
;
Yuan CAO
3
;
Guan-lin YANG
3
Author Information
1. The First Affiliated Hospital of Liaoning University of Traditional Chinese Medicine(TCM), Shenyang 110032, China
2. Affiliated Stomatological Hospital of China Medical University, Shenyang 110031, China
3. Key Laboratory of TCM Viscera Theory and Application, Ministry of Education, Liaoning University of TCM, Shenyang 110847, China
- Publication Type:Research Article
- Keywords:
Huayu Qutan decoction;
vascular dementia;
calcium ion-calmodulin-dependent protein kinase Ⅱ (CaMKⅡ);
cyclic adenosine effect element binding protein (CREB);
brain-derived neurotrophic factor (BDNF);
Si Junzitang
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(16):31-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the protective effect of Huayu Qutan decoction on vascular dementia (VD) gerbils and to explore whether its mechanism is related to Calcium ion-calmodulin-dependent protein kinase Ⅱ (CaMKⅡ)/cyclic adenosine effect element binding protein (CREB)/brain-derived neurotrophic factor (BDNF) signaling pathway. Method: Forty healthy gerbils were randomly divided into sham operation group, model group, low, medium and high dose groups (5.35, 10.7, 21.4 g·kg-1) of removing blood stasis and expelling phlegm. Eight gerbils in each group were divided into model group and removing blood stasis and expelling phlegm group. Gerbils were given corresponding drugs twice a day after operation. Water maze experiment was conducted 21 days later to investigate the spatial learning and memory ability of gerbils. The expression of p-CaMKⅡ/CaMKⅡ, p-CREB/CREB and BDNF in the hippocampus of gerbils were detected by Western blot and immunohistochemistry. Result: Compared with sham operation group, the incubation period and the number of platform trips of gerbil in the model group were significantly reduced, p-CaMKⅡ/CaMKⅡ, p-CREB/CREB, and BDNF protein expression were significantly reduced (P<0.01). Compared with model group, the incubation period and the number of platform shuttling of gerbil in Huayu Qutan decoction group were significantly increased, among which the high-dose phlegm dispelling prescription had the most significant effect (P<0.01). The phlegm dispelling prescription could effectively protect pyramidal cells in CA1 region, significantly up-regulating the expression of p-CaMKⅡ/CaMKⅡ, p-CREB/CREB and BDNF protein (P<0.01). Conclusion: Huayu Qutan decoction improves the learning and memory abilities of gerbils with vascular dementia, and its mechanism may be related to the activation of CaMKⅡ/CREB/BDNF signaling pathway.
