Protective Effect of Danggui Buxue Tang on Impaired Functional Activity of Endothelial Cells Exposed to Low Flow Shear Stress
10.13422/j.cnki.syfjx.20191602
- VernacularTitle: 当归补血汤对低流体剪切应力作用下内皮细胞功能损伤的保护作用
- Author:
Zhen QIN
1
;
Zheng-xin WEI
1
;
Shui-qing HUANG
2
Author Information
1. School of Basic Medicine, Guizhou Medical University, Guiyang 550025, China
2. School of Extended Education, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
- Publication Type:Research Article
- Keywords:
Danggui Buxue Tang;
fluid shear stress;
endothelial cell;
functional activity;
nitric oxide
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(16):12-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the protective effect of Danggui Buxue Tang(DGBX)with Angelicae Sinensis Radix(AS) and Astragali Radix(AR)at different radios on impaired functional activity of endothelial cells(ECs)exposed to low-fluid shear stress (FSS). Method: Low FSS was loaded by a parallel plate flow chamber,and ECs were divided into normal FSS group,low FSS group(each preincubated with M199 medium for 2 h),simvastatin group(preincubated with 0.1 μmol·L-1 simvastatin for 2 h),and 3 DGBX groups(preincubated with 3 g·L-1 AS and AR at 1:1,1:3,1:5 for 2 h,respectively). Then,the normal group was exposed to 1.2 Pa FSS,while the rest groups were all exposed to low FSS. At time points of 30,60, 360 min,the proliferation was detected by methyl thiazoly tetrazolium(MTT),the secretion of nitric oxide(NO) was detected by nitrase reduction test,and the mRNA and protein expressions of endothelial nitric oxide synthase(eNOS) were detected by Real-time fluorescence guantitative polymerase chain reaction(Real-time PCR) and Western blot,respectively. Result: Compared with the normal group, the secretion of NO and the expression of eNOS in ECs were both increased significantly at 60 min (P<0.01),then decreased at 360 min. Compared with the model group,there was no significant change in proliferation in DGBX groups,but DGBX could promote the NO secretion and the expression of eNOS in ECs exposed to low FSS at 360 min respectively, whereas the function of DGBX(AS and AR at 1:3,1:5) was obviously observed. Conclusion: DGBX could protect the functional activity of ECs exposed to low FSS.
