Effect of Shaoyaotang on Expressions of AP-1 and TNF-α in Colon of Rats with Hygrothermal Ulcerative Colitis
10.13422/j.cnki.syfjx.20191603
- VernacularTitle: 芍药汤对湿热内蕴型溃疡性结肠炎大鼠模型结肠组织中AP-1，TNF-α表达的影响
- Author:
Feng-yi WANG
1
;
Dang-sheng ZHAO
1
;
Xiao-wei PU
1
;
Jian ZU
1
;
Min XU
1
;
Chen-an SUN
1
Author Information
1. Gansu University of Chinese Medicine, Lanzhou 730000, China
- Publication Type:Research Article
- Keywords:
Shaoyaotang;
ulcerative colitis;
activator protein-1 (AP-1);
tumour necrosis factor (TNF-α)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(16):7-11
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of Shaoyaotang on mRNA and protein expressions of colon tissue activated protein-1 (AP-1) and tumor necrosis factor-α (TNF-α) of hot and humid-type intrinsic ulcerative colitis (UC) model in rats, in order to explore the mechanism of action of herbaceous peony decoction in the treatment of UC. Method: Totally 60 Wistar rats were randomly divided into blank group, model group, SASP group, and low, medium and high-dose Shaoyaotang groups. The damp-heat intrinsic UC rat model was replicated based on integrated disease and syndrome, namely, high-fat and high-sugar spicy food and immune complex method combined with 2,4,6-trinitrobenzene sulfolnic acid (TNBS) and ethanol complex method. After the successful modeling, low, medium and high-dose Shaoyaotang (6, 12, 24 g·kg-1) was given by gavage, and 1 g·kg-1 dose of salazol sulfadiazine was given to by gavage. The blank group was given constant volume normal saline for 21 d. Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR) was used to detect mRNA expressions of AP-1 and TNF-α in colon tissues, and Western blot was used to detect protein expressions of AP-1 and TNF-α in colon tissues. Result: Compared with the blank group, relative mRNA and protein expressions of AP-1, TNF-α in the model group were significantly increased (P<0.05). Compared with the model group, mRNA and protein expressions of AP-1, TNF-α in the treatment groups were significantly decreased (P<0.05). Conclusion: Shaoyaotang can inhibit the expression of TNF-α and stimulate AP-1 protein expression in rats with damp-heat UC.
