Quality Evaluation of Galli Gigerii Endothelium Corneum by Allele-specific PCR Method
10.13422/j.cnki.syfjx.20191717
- VernacularTitle: 基于物种特异性PCR方法的鸡内金真伪鉴别
- Author:
Jing-zhe PU
1
;
Ya-zhong ZHANG
1
;
Ye-lin ZHU
2
;
Chao JIANG
3
;
Yuan YUAN
3
Author Information
1. Anhui Institute for Food and Drug Control, Hefei 230051, China
2. Anhui Provincial Market Supervision Administration, Hefei 230051, China
3. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia, China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
Galli Gigerii Endothelium Corneum;
duck Gizzard Membrane;
goose Gizzard Membrane;
adulterants
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(17):142-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To screen the specific reverse primers of Galli Gigerii Endothelium Corneum,duck gizzard membrane and goose gizzard membrane,and establish a specific PCR for molecular identifying Galli Gigerii Endothelium Corneum and its common adulterants. Method: Based on the mutation sites on the 12S rRNA sequence,specific polymerase Chain reaction(PCR) identify primers were designed for chicken,duck and goose gizzard membrane. The specific PCR reaction conditions were optimized,and the PCR identification method was explored and verified in terms of tolerance and feasibility. Thirty batches of Galli Gigerii Endothelium Corneum decoction pieces extracted from the test were identified. Result: Thirty batches of Galli Gigerii Endothelium Corneum decoction pieces were detected using chicken-specific primers, 273 bp of specific bands was amplified and visualized on the agarose electrophoregram. When duck and goose primers were used,no corresponding amplified band was detected. Conclusion: The allele-specific PCR method can be used as a rapid and accurate method to identify Galli Gigerii Endothelium Corneum. It is a promise method for special sampling tasks of Chinese herbal medicine and decoction tablets nationwide.
