Specific PCR Method for Identification of Suis Fellis Pulvis and Its Chinese Patent Medicines
10.13422/j.cnki.syfjx.20191714
- VernacularTitle: 猪胆粉及其中成药的特异性PCR鉴别方法
- Author:
Li WANG
1
;
Yan JIN
1
;
Chao JIANG
1
;
Yuan YUAN
1
Author Information
1. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
Suis Fellis Pulvis;
Chinese patent medicine;
specific polymerase Chain reaction(PCR);
molecular identification
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(17):136-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective: A polymerase Chain reaction(PCR) identification method for Suis Fellis Pulvis and its Chinese patent medicines was established to provide an example for the identification of animal-derived components in complex components. Method: A PCR identification method was established based on swine derivatives identification primers,the reaction system was optimized,and the established method was investigated and verified. By the established PCR identification method,the swine derivatives of 20 batches of self-made Suis Fellis Pulvis material,19 batches of commercially available Suis Fellis Pulvis and 22 batches of Chinese patent medicines containing Suis Fellis Pulvis were identified. The commercially available Suis Fellis Pulvis material and Chinese patent medicines containing Suis Fellis Pulvis positive products that were amplified PCR were verified by enzyme digestion and sequencing. Result: Totally 20 batches of self-made Suis Fellis Pulvis material and Suis Fellis Pulvis control material could expand the specific identification band of about 212 bp,and there was no bands in bovine and ovine reference, only 5 batches of the 19 batches of commercially available Suis Fellis Pulvis had expanded specific identification bands, 10 batches of 22 batches of Chinese patent medicines containing Suis Fellis Pulvis were detected to have swine derivatives, the Suis Fellis Pulvis control material and the PCR-amplified commercially available Suis Fellis Pulvis material positive products can produce about 200 bp of bands after digestion with Mnl I. The highest similarity between the amplification products sequence of Suis Fellis Pulvis and its Chinese patent medicines, and the GenBank database was Sus scrofa,the consistency was 99%,which conformed to the sequence of swine. Conclusion: The PCR identification method established in this paper can accurately identify the biological origin of Suis Fellis Pulvis and its Chinese patent medicines.
