Effect of Berbamine Hydrochloride on Intestinal Absorption Characteristics of Berberine Hydrochloride by Rat Everted Intestinal Sac Model
10.13422/j.cnki.syfjx.20190749
- VernacularTitle: 外翻肠囊法考察盐酸小檗胺对盐酸小檗碱肠吸收特性的影响
- Author:
Yu-nan XIANG
1
;
Xiao-yan WANG
1
;
Hui FENG
1
;
Ya ZHAO
1
;
Qi-en LI
2
;
Xian-jia LI
2
;
Xian-rong LAI
1
Author Information
1. College of Pharmacy, School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
2. Tibetan Medical College, Qinghai University, Xining 810000, China
- Publication Type:Research Article
- Keywords:
berberine hydrochloride;
berbamine hydrochloride;
everted intestinal sac method;
high performance liquid chromatography(HPLC);
intestinal absorption;
P-glycoprotein;
high calcium environment
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(17):98-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of berbamine hydrochloride on the absorption characteristics of berberine hydrochloride in different intestinal segments of rats in normal environment and high calcium environment. Method: Taking rat everted intestinal sac model,the content of berberine hydrochloride in absorbent solution of everted intestinal sac from different compatibility groups was determined by HPLC,and the uptake per unit area in different groups was analyzed by One-way ANOVA. Result: Compared with the normal J70 group(in normal environment,the concentration of berberine hydrochloride was 70 mg·L-1) at the same time point,the uptake per unit area of the normal J70+Ver100 group(in normal environment,the concentration of berberine hydrochloride was 70 mg·L-1,adding verapamil hydrochloride to a concentration of 100 mg·L-1) was significantly increased in the ileum(P<0.05) at 120 min;the uptake per unit area values of the high calcium J70+A35 group(in high calcium environment,the concentration of berberine hydrochloride was 70 mg·L-1,adding berbamine hydrochloride to a concentration of 35 mg·L-1) were significantly increased in the duodenum(P<0.05) at 30,60,90 min;and the uptake per unit area values of the high calcium J70+A70 group(in high calcium environment,the concentration of berberine hydrochloride was 70 mg·L-1,adding berbamine hydrochloride to a concentration of 70 mg·L-1) were significantly increased in the ileum(P<0.05) at 30,90,120 min.By comparing with the normal group with the same mass concentration,the intestinal absorption of berberine hydrochloride was better in the high calcium J70+A35 group and the high calcium J70+A70 group. Conclusion: Berbamine hydrochloride can promote the absorption of berberine hydrochloride in intestine to a certain extent,especially in the high calcium environment.
