Effect of Compound Danshen Dropping Pills on Heart of Diabetic Cardiomyopathy Rats by Regulating VEGF/TGF-β1 by miRNA-200b
10.13422/j.cnki.syfjx.20191606
- VernacularTitle: 复方丹参滴丸通过miR-200b调控VEGF/TGF-β1影响糖尿病心肌病大鼠心脏的作用机制
- Author:
Ya-wen FAN
1
;
Li-chang LIANG
1
;
Tian-feng ZHANG
1
;
Lei MU
1
;
Tian XIE
1
;
Xiao-bing JIANG
2
;
Hui REN
2
Author Information
1. Shenzhen Hospital of Guangzhou University of Chinese Medicine(Futian), Shenzhen 518034, China
2. The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
- Publication Type:Research Article
- Keywords:
compound Danshen dropping pills;
diabetic cardiomyopathy;
miR-200b;
vascular endothelial growth factor(VEGF);
transforming growth factor(TGF)-β1;
Collagen Ⅰ
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(17):34-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the protective effect and mechanism of compound Danshen dropping pills on the heart of rats with type 2 diabetic cardiomyopathy. Method: Seventy-five male SD rats were randomly divided into blank group (15 rats) and model group (60 rats). The model group was fed with high-sugar and high-fat diet, high-sugar and high-fat milk and sucrose water. Six weeks later, rats with foodborne obesity (body mass ≥ the mean body mass of blank control group + 2 times standard deviation) were selected and intraperitoneally injected with 1% streptozotocin (STZ)28 mg·kg-1. After 12 weeks, rats with fasting blood glucose ≥ 11.1 mmol·L-1 and polydipsia, diuresis, polyphagia and weight loss were randomly divided into model group, compound Danshen dropping pills group (0.5 g·kg-1) and metformin group (0.5 g·kg-1) and intervened for 12 weeks. At the end of the experiment, echocardiography was used to evaluate cardiac function. Blood samples were taken to determine the corresponding biochemical indicators of rats. Histopathological changes of myocardium were observed by hematoxylin-eosin (HE) and Masson staining. The ultrastructure of myocardium was observed under electron microscope. Western blot was used to detect the expressions of vascular endothelial growth factor (VEGF), transforming growth factor-beta 1 (TGF-β1) and type Ⅰ collagen (Collagen Ⅰ). Real-time fluorescence quantitative analysis (Real-time PCR) was used to detect the expression of miRNA-200b. Result: Compared with the blank group, the heart weight index (HMI), fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c), fasting insulin (FINS), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterin(LDL-C) levels in the model group were significantly increased (P<0.05, P<0.01), while the levels of high-density lipoprotein cholesterin(HDL-C) decreased significantly (P<0.01); the cardiac function was impaired, the deposition of collagen fibers increased (P<0.01), and the degree of myocardial tissue damage was obvious; the expression of miR-200b in myocardial tissue decreased (P<0.01), and the expressions of VEGF, TGF-β1 and Collagen I increased (P<0.01). Compared with the model group, the levels of HMI, TC, TG and LDL-C in the compound Danshen dropping pills group were significantly increased (P<0.05, P<0.01), and the levels of HDL-C increased significantly (P<0.01), the cardiac function was improved, the deposition of collagen fibers in myocardium was decreased (P<0.05), the degree of myocardial tissue damage was reduced, the expression of miRNA-200b in myocardium was increased (P<0.05), and the expressions of VEGF, TGF-β1 and Collagen I was decreased (P<0.05, P<0.01). Conclusion: Compound Danshen dropping pills may play a protective role in the heart of type 2 diabetic cardiomyopathy rats by lowering lipid, up-regulating the expression of miR-200b and inhibiting the expression levels of VEGF, TGF-β1 and Collagen I.
