Effect of Manual and Machine Decocting on Chemical Constituents in Bazhentang Based on Non-targeted Metabolomics
10.13422/j.cnki.syfjx.20190850
- VernacularTitle: 基于非靶向代谢组学分析手工与机器煎药对八珍汤中化学成分的影响
- Author:
Jun-yi ZHOU
1
;
Yu LIN
2
;
Qiong LUO
3
;
Yao-zhou HUANG
4
;
Na LANG
4
;
Xiong WANG
5
;
Yong-liang ZHAO
2
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. Beijing HUANG Yaozhou Research Institute of Chinese Medicine Co. Ltd., Beijing 100142, China
3. China Institute for History of Medicine and Medical Literature, China Academy of Chinese Medical Sciences, Beijing 100700, China
4. Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
5. Miyun District Hospital of Traditional Chinese Medicine, Beijing 101599, China
- Publication Type:Research Article
- Keywords:
Bazhentang;
manual decocting;
machine decocting;
non-targeted metabolomics;
differential components;
senkyunolide A;
electrospray ionization
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(17):7-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the effect of manual decocting and machine decocting on the chemical constituents in Bazhentang based on non-targeted metabolomics, and to find the differential chemical constituents of these two decocting methods. Method: Bazhentang was boiled by standardized manual decocting and machine decocting methods,respectively. Orthogonal partial least square-discriminate analysis(OPLS-DA) and other multivariate statistical methods, combined with variable importance in the projection(VIP) value and t-test, were employed to analyze the effect of two decocting methods on the chemical constituents in Bazhentang. The differential chemical constituents were analyzed by UPLC-LTQ-Orbitrap MS under positive and negative ion modes,mobile phase was acetonitrile-0.1%formic acid aqueous solution for gradient elution,the scanning range was m/z 50-1 500. Result: Under the positive and negative ion modes of high-resolution mass spectrometry, a total of 87 differential components were found,40 of them were identified according to the mass spectrometry data and literature reports, including senkyunolide A, glycyrrhizin, ferulic acid, etc. Conclusion: Based on the analysis of color and chemical compositions of Bazhentang, there are obvious differences between the standardized manual decocting and machine decocting. If the advantages of these two methods are combined,a standardized decoction process can be established on the basis of maintaining the advantages of manual decocting, the effectiveness of traditional Chinese medicine decoction will be maximized and it will be convenient for patients to take it.
