Determination of Biflavones in Ginkgo biloba Leaves of Different Ages by UPLC-TQ-MS
10.13422/j.cnki.syfjx.20191414
- VernacularTitle: 基于UPLC-TQ-MS考察不同树龄银杏叶双黄酮含量变化规律
- Author:
Dan-hong YU
1
;
Wei JIANG
2
;
Xin YAO
3
;
Ping XUE
4
;
Chen-mei MAO
1
Author Information
1. Children's Hospital of Soochow University, Suzhou 215000, China
2. Taizhou Institute for Food and Drug Control, Taizhou 225300, China
3. The First Affiliated Hospital of Soochow University, Suzhou 215000, China
4. Changzhou Institute for Food and Drug Control, Changzhou 213000, China
- Publication Type:Research Article
- Keywords:
UPLC-TQ-MS;
Ginkgo biloba leaves;
biflavones;
tree age
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(18):145-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an ultra-high performance liquid chromatography coupled with triple quadrupole mass for the determination of five biflavones. Method: Chromatographic separation was carried out on a ACQUITY UPLC BEH C18 column (2.1 mm×100 mm,1.7 μm) with gradient elution of acetonitrile and 0.10%formic acid at a flow rate of 0.3 mL·min-1,and the column temperature was set at 35℃. Result: Amentoflavone,bilobetin,ginkgetin,isoginkgetin,sciadopitysin showed a good linearity within the ranges of 0.02-13.20 mg·L-1(r=0.996 3),0.05-23.60 mg·L-1(r=0.995 5),0.09-18.60 mg·L-1(r=0.992 7),0.10-21.00 mg·L-1(r=0.998 8),0.06-16.00 mg·L-1(r=0.996 7),with average recoveries of 101.50%,98.78%,97.59%,97.24%,101.09%, and RSDs of 2.7%,2.7%,3.1%,2.8%,1.3%. The contents of amentoflavone,bilobetin,ginkgetin,isoginkgetin,sciadopitysin ranged between 121.30-434.74,268.39-847.14,251.80-1 297.10,195.87-691.10,477.48-3 003.90 μg·g-1. The total biflavones ranged between 1 474.45-5 635.40 μg·g-1. It shows a certain regularity that the low-vinegar leaves contain higher total flavonoids,and the total flavonoid content gradually decreases with the increase of tree age. Conclusion: The method was simple, and can be used for qualitative and quantitative analysis of biflavones.
