Effect of Erxian Decoction on Cardiac Electrophysiology in Peri-menopausal Rats
10.13422/j.cnki.syfjx.20191601
- VernacularTitle: 二仙汤对围绝经期大鼠心脏电生理的影响
- Author:
Hui-hui XIE
1
;
Ying WANG
1
;
Shuo YANG
1
;
Ye-hong DONG
1
;
Li ZHANG
1
;
Si-min HUANG
1
;
Peng XU
1
;
Bo LIU
1
Author Information
1. College of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
Erxian decoction;
peri-menopause;
estrogen;
cardiac electrophysiology
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(18):43-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect and mechanism of Erxian decoction on peri-menopausal cardiac electrophysiology in rats. Method: Female sprague-dawley rats were randomly divided into six groups:sham operation group, model group, estradiol valerate group, and low, medium and high-dose Erxian decoction groups. Except the sham operation group, the rats in the other groups were completely removed from the ovarian replication peri-menopausal rat model. At the same time, estradiol valerate group (8×10-4 g·kg -1·d-1), low-dose Erxian decoction group (4 g·kg-1·d-1), middle-dose Erxian decoction group (8 g·kg-1·d-1) and high-dose Erxian decoction group (12 g·kg-1·d-1), sham operation group and model group were given the same amount of normal saline. The administration was given once a day for 80 consecutive days. The electrocardiogram of rats was recorded by biosignal detector connected to lead Ⅱ electrode. The content of estradiol (E2) in rat serum was detected by enzyme-linked immuno sorbent assay (ELISA). The pathological changes of rat uterus were observed by hematoxylin-eosin. The expression of the estrogen alpha receptor (ERα) protein was detected by Western blot method in myocardial tissue of rat. Result: Compared with the sham operation group, the amplitudes of P wave, R wave and T wave in the electrocardiogram of the model group were significantly decreased (P<0.01), and the PR interval, QRS time course and QT interval were shortened to different degrees. The E2 level was significantly lower (P<0.01). The uterus was obviously shriveled and shrank, the endometrial layer became thinner, and the number of glands reduced significantly. The expression of ERα receptor protein in myocardial tissue was significantly decreased (P<0.01). Compared with the model group, the Erxian decoction group could improve the pathological changes of ECG in the above rats. The medium and high-dose Erxian decoction can significantly increase the E2 level (P<0.01), reverse significantly the pathological changes of the uterus, and up-regulate the expression of ERα receptor protein (P<0.01). Conclusion: Erxian decoction can improve cardiac electrophysiological changes in peri-menopausal rats, and its mechanism may be related to the increase of estrogen activity and estrogen receptor expression in rats.
