Mechanism of Huangliantang in Treatment of Chronic Non-atrophic Gastritis Based on Harmonization Method
10.13422/j.cnki.syfjx.20191702
- VernacularTitle: 基于和法探讨黄连汤治疗慢性非萎缩性胃炎的机制
- Author:
Guang-zhi LUO
1
;
Cheng-en HAN
2
;
Xiao-chun HAN
1
;
Yuan WANG
1
;
Ting MA
1
;
Shi-jun WANG
1
;
Cheng-bo ZHANG
3
Author Information
1. Shandong University of Traditional Chinese Medicine(TCM), Ji'nan 250355, China
2. Affiliated Hospital of Shandong University of TCM, Ji'nan 250011, China
3. Shandong Co-innovation Center of Classic TCM formula, Ji'nan 250355, China
- Publication Type:Research Article
- Keywords:
harmonizing method;
Huangliantang;
chronic non-atrophic gastritis;
nuclear factor-κB (NF-κB)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(18):36-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Harmonization method is one of the eight unique methods of traditional Chinese medicine with important application value in clinic. Based on the effect of harmonization method in regulating cold and heat, the mechanism of Huangliantang in treating chronic non-atrophic gastritis(CNAG) on rats were studied. Method: Rats were divided into normal group (n=10) and CNAG model group (n=50). The model of CNAG rats was induced by chemical stimulation combined with hunger and satiety. The model group was randomly divided into 5 groups, namely the model group, the Jinghua Weikang pill treatment group, and the high, middle and low-dose Huangliantang groups, with 8 rats in each group. After the model was successfully established, the Jinghua Weikang pill treatment group (0.04 g·kg-1), the high, middle, low dose Huangliantang group (11.00,5.48,2.74 g·kg-1), the blank group and the model group were given the same dose of saline for 4 weeks, and then the samples were collected. The histological changes of gastric mucosa were observed by hematoxylin-eosin (HE) staining. The serum levels of interleukin-6 (IL-6), interleukin-1β (IL-1β) and interleukin-8 (IL-8) were measured by enzyme-linked immunosorbent assay (ELISA). immunohistochemistry(IHC) was used to detect nuclear factor-κB (NF-κB) and its inhibitory protein receptor (IκBα), protein expression. Real-time quantitative fluorescence polymerase chain reaction (Real-time PCR) was used to detect IκBα, NF-κB mRNA expressions. Result: In the model group, the gastric mucosa was damaged, a large number of inflammatory cells were infiltrated, the serum inflammatory factors increased significantly, mRNA and protein expressions of IκBα decreased, and mRNA and protein expressions of NF-κB increased in the gastric tissue (P<0.01). In each treatment group, the inflammation of gastric mucosa was improved to some extent, the serum inflammatory factor was decreased, the mRNA expression of IκBα was up-regulated, IκBα protein was increased, while the expression of NF-κB mRNA was down-regulated, and NF-κB protein was decreased. The Jinghua Weikang pill treatment group and the high-dose Huangliantang group had the most obvious improvement (P<0.05, P<0.01). Conclusion: Huangliantang for regulating cold and heat based on the harmonization method can effectively alleviate the degree of gastric mucosal injury, and reduce serum inflammatory factors in CNAG rats. The mechanism is related to the up-regulation of IκBα mRNA expression, and the down-regulation of NF-κB mRNA expression and NF-κB protein expression in gastric mucosa.
