Review of Zuoguiwan on Animal Models and Evaluation in Prevention and Treatment of Osteoporosis
10.13422/j.cnki.syfjx.20191525
- VernacularTitle: 左归丸防治骨质疏松症的动物模型和评价方法
- Author:
Fei-xiang LIU
1
;
Zi-xuan LIN
1
;
Dao-ming XU
2
;
Su-min YE
1
;
Ming-yue ZHANG
1
;
Qiao-ling FAN
1
;
Feng TAN
1
;
Xing LI
1
;
Yi CHAI
1
;
Wen-bin SHANG
1
;
Sheng-feng LU
1
Author Information
1. College of Basic Medicine, First School of Clinical Medicine, Key Laboratory of Acupuncture and Medicine Research Under Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210023, China
2. Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China
- Publication Type:Research Article
- Keywords:
Zuoguiwan;
postmenopausal osteoporosis;
animal models;
evaluation methods
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(18):16-23
- CountryChina
- Language:Chinese
-
Abstract:
Zuoguiwan is a classic prescription for replenishing vital essence, tonifying kidney-Yin and nourishing the bone marrow. Zuoguiwan is one of the effective prescriptions for the prevention and treatment of osteoporosis (OP), which reflects the thought of Reinforcing Yang from Yin. The OP animal model simulates the pathological state and pathogenesis of OP in human, which is an important means to research the pathogenesis of OP and verify the effect of drugs. In this paper, two kinds of animal models and characteristics of Zuoguiwan in the prevention and treatment of osteoporosis were discussed in details. They are the primary osteoporosis animal models, including ovariectomized animal models and spontaneous elderly osteoporosis animal models, and the secondary osteoporosis models, including glucocorticoid-induced animal models, cyclophosphamide-induced animal models and subtotal nephrectomy animal models. The evaluation methods of Zuoguiwan in preventing and treating OP, including bone absorption markers and bone formation markers analyzed by enzyme-linked immunosorbent assay (ELISA), bone mineral density detected with dual-energy X-ray, the number of trabeculae, trabecular segregation, trabecular thickness, bone volume/tissue volume ratio and bone surface/volume ratio analyzed using micro-CT, bone pathological morphology observed by hematoxylin-eosin staining, bone biomechanical properties, such as the maximum load force based on biomechanical test. In order to provide scientific reference for the basic and clinical research of Zuoguiwan, the OP animal models and the pharmacodynamic effect of Zuoguiwan are evaluated comprehensively with five different and objective evaluation methods. However, the animal model of OP needs to be further optimized to highlight the pathogenesis and syndrome characteristics of Zuoguiwan in the treatment of OP.
