Effect of Zuoguiwan in Improving BMD and Trabecular Bone Microarchitecture in Ovariectomy-induced Osteoporosis Rats by Regulating RANKL/OPG Pathway Mediated by β2AR
10.13422/j.cnki.syfjx.20191524
- VernacularTitle: 左归丸调节β2AR介导的RANKL/OPG信号通路改善去卵巢大鼠骨量和显微结构水平
- Author:
Fei-xiang LIU
1
;
Zi-xuan LIN
1
;
Dao-ming XU
2
;
Su-min YE
1
;
Ming-yue ZHANG
1
;
Qiao-ling FAN
1
;
Feng TAN
1
;
Xing LI
1
;
Yi CHAI
1
;
Cong-ying MAI
1
;
Sheng-feng LU
1
Author Information
1. Nanjing University of Chinese Medicine, Nanjing 210023, China
2. Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China
- Publication Type:Research Article
- Keywords:
Zuoguiwan;
osteoprotegerin;
receptor activator of nuclear factor kappa-B ligand(RANKL);
β2-adrenergic receptor(β2AR);
postmenopausal osteoporosis(OPG)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(18):1-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the mechanism of Zuoguiwan in treating ovariectomy-induced osteoporosis rats by receptor activator of nuclear factor kappa-B ligand (RANKL)/osteoprotegerin (OPG) signaling pathway mediated by β2-adrenergic receptor (β2AR). Method: Forty Sprague-Dawley female rats were randomly divided into Sham-operated group (Sham) and four ovariectomized (OVX) subgroups. Rats in Sham and OVX groups were treated with 17β-estradiol (50 μg·kg-1·d-1), and low and high-dose ZGW (2.3,4.6 g·kg-1 lyophilized powder) for 3 months, respectively. Enzyme-linked immunosorbent assay (ELISA) was used to measure the serum markers of bone turnover. Micro-CT was used to evaluate and measure trabecular bone microarchitecture and bone mineral density (BMD) of the right distal femur. Western blot analysis and Real-time PCR were used to measure mRNA and protein expressions of β2AR, OPG and RANKL. Result: After 12 weeks of treatment with Zuoguiwan, the level of serum β-cross-linked c-telopeptide of type Ι collagen (β-CTX) (P<0.01) was lower, while the level of serum bone-specific alkaline phosphatase (BALP) was higher (P<0.01) than those in the OVX group. Moreover, it could prevent the OVX-induced bone loss, and alleviate the trabecular bone microarchitecture of distal femur. Furthermore, Zuoguiwan could up-regulate the mRNA and protein expressions of OPG in tibia of the Zuoguiwan groups(P<0.01), reduce the mRNA and protein expressions of β2AR in the hypothalamus (P<0.01), and down-regulated the mRNA and protein expressions of RANKL (P<0.05) in the tibia, compared with those in the OVX group. Conclusion: The mechanism of Zuoguiwan in alleviating BMD and trabecular bone microarchitecture in ovariectomy-induced osteoporosis rats might be related to the regulation of RANKL/OPG Pathway mediated by β2AR.
