Content Changes of Eight Components in Tetrastigmatis Hemsleyani Radix of Different Months
10.13422/j.cnki.syfjx.20191111
- VernacularTitle: 不同月份三叶青中8种有效成分含量变化
- Author:
Shi-min LI
1
;
Chong-lu SUN
1
;
Yu-jiong ZHANG
1
;
Yuan SUN
1
;
Xin PENG
1
Author Information
1. Zhejiang Pharmaceutical College, Ningbo 315100, China
- Publication Type:Research Article
- Keywords:
Tetrastigmatis Hemsleyani Radix;
different months;
chlorogenic acid;
rutin;
polydatin
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(19):117-123
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a HPLC method for determination of chlorogenic acid,rutin,polydatin,kaempferol-3-O-rutinoside,astragalin,resveratrol,quercetin,kaempferol in Tetrastigmatis Hemsleyani Radix,in order to study the content changes of eight components of different months. Method:Zorbax SB C18column (4.6 mm×250 mm,5 μm)was adopted with mobile phase consisting of acetonitrile(A)and 0.1%phosphoric acid(B)in gradient elution(0-30 min,10%-30%A;30-40 min,30%-95%A;40-45 min,95%A;45-60 min,95%-10%A). The flow rate was 0.8 mL·min-1,the column was kept at 25℃,and the detection wavelength was 320 nm. Result:Chlorogenic acid,rutin,polydatin,kaempferol-3-O-rutinoside,astragalin,resveratrol,quercetin,kaempferol showed a good linearity within the range of 13.7-549 mg·L-1(r=0.999 0),12.6-253 mg·L-1(r=0.999 1),15.8-316 mg·L-1(r=0.999 0),14.7-147 mg·L-1(r=0.999 2),8.8-88 mg·L-1(r=0.999 1),7.9-79 mg·L-1(r=0.999 5),8.6-172 mg·L-1(r=0.999 1),8.9-89 mg·L-1(r=0.999 4). There were great differences in contents of the eight flavonoid active components in different growth phases. In July and August,the relative contents of chlorogenic acid,rutin,kaempferol 3-O-rutinoside and polydatin were the highest. The highest relative content of quercetin was observed in June. The relative contents of resveratrol and kaempferol in April and May was higher than those in other mouths. The relative content of astragalin in November was the highest. Conclusion:It could provide abundant information for the production and quality control of Tetrastigmatis Hemsleyani Radix.
