Rapid Identification of Alkaloids in Evodia rutaecarpa by HPLC-Q-TOF-MS/MS
10.13422/j.cnki.syfjx.20190915
- VernacularTitle: HPLC-Q-TOF-MS/MS快速鉴别吴茱萸中的生物碱类组分
- Author:
Xiao-long HUANG
1
;
Bing-bing SHEN
2
;
Xue-juan LIANG
2
;
Shu-yun SHI
3
;
Du-zhun ZOU
1
;
Yu-ting ZENG
1
;
Jing-kun QU
1
;
Shui-han ZHANG
2
Author Information
1. Hunan University of Chinese Medicine, Changsha 410208, China
2. Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha 410013, China
3. Chemistry and Chemistry Engineering, Central South University, Changsha 410083, China
- Publication Type:Research Article
- Keywords:
Evodia rutaecarpa;
alkaloids;
indole alkaloids;
quinolone alkaloids;
ephedrine
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(19):102-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain the information of alkaloids in Evodia rutaecarpa by HPLC-Q-TOF-MS/MS. Method:Inter Sustain-C18 column (4.6 mm×250 mm,5 μm) was used with 0.2% formic acid water-acetonitrile as the mobile phase for gradient elution. The column temperature was 25℃,the volume flow rate was 1.0 mL·min-1,and the sample volume is 5 μL. The detection wavelength was 245 nm,and the chromatographic effluent was detected and analyzed by using both positive and negative ions. Result:According to molecular ion peaks and secondary mass spectrometry characteristic fragment ions,as well as the mass spectrometry information of reference substances and relevant literature reports,more than 40 major peaks were analyzed,and 21 alkaloids were identified from the methanol extract of E. rutaecarpa, including 10 kinds of indole alkaloids,10 kinds of quinolone alkaloids,and 1 kind of ephedrine. Main types of alkaloids in E. rutaecarpa were basically clarified. And the research found that the alkaloids have a good response mainly in the positive mode. Conclusion:Based on HPLC-Q-TOF-MS/MS technology, high-performance liquid chromatography (HPLC) separation,mass spectrometry determination of molecular mass,pyrolysis data,literature analysis and retrieval were performed to quickly,accurately and comprehensively identify alkaloids in E. rutaecarpa, so as to provide a scientific basis for the further extraction and separation of the chemical constituents of E. rutaecarpa.
