Effect of Mangiferin on Morphology of Relevant Vessels and MCP-1/CCR-2 Pathway in Spontaneously Hypertensive Rats
10.13422/j.cnki.syfjx.20192039
- VernacularTitle: 芒果苷对自发性高血压大鼠相关血管形态学及MCP-1/CCR-2通路的影响
- Author:
Cheng-pu LIAO
1
;
Xue-wen ZENG
1
;
Tian-tian XIN
1
;
Yu-mei MENG
1
;
Da-li HUANG
1
;
Xiao-qin HU
1
Author Information
1. Guangxi University of Traditional Chinese Medicine(TCM), Guangxi Key Laboratory of Pharmacodynamics of TCM, Nanning 530001, China
- Publication Type:Research Article
- Keywords:
mangiferin;
spontaneously hypertensive rats;
related blood vessels;
morphology;
monocyte chemoattract protein-1 (MCP-1)/c-chemokine receptor type 2 (CCR-2) pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(19):39-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the morphological changes of carotid artery, thoracic aorta and superior mesenteric artery in spontaneously hypertensive rats(SHR), in order to further study the effect of Mangiferin on the expressions of inflammatory factors and monocyte chemoattract protein-1 (MCP-1)/c-chemokine receptor type 2 (CCR-2) pathway in SHR. Method:Forty spontaneously hypertensive rats were randomly divided into model group, benazepril group (10 mg·kg-1·d-1) and low, medium and high-dose mangiferin groups (25, 50, 100 mg·kg-1·d-1). Eight male WKY rats of the same age were selected as normal control group. Systolic blood pressure was observed every two weeks after eight weeks of administration. Morphology of carotid artery, thoracic aorta and superior mesenteric artery was observed by hematoxylin-eosin (HE) staining. Immunohistochemical assay (IHC) and Western blot were used to detect MCP-1 and CCR-2 protein expressions in thoracic aorta. MCP-1 and CCR-2 mRNA expression levels in thoracic aorta were detected by Real-time quantitative fluorescence PCR (Real-time PCR). Result:Compared with the normal group, the inflammatory cells in the model group increased significantly, the systolic blood pressure was significantly higher than that in the WKY group (P<0.01), and MCP-1, CCR-2 protein and mRNA expressions in thoracic aorta were increased obviously (P<0.01). Compared with model group, in high-dose benazepril and mangiferin groups, inflammatory cells were decreased significantly, endothelial margin and fibrous tissue infiltration were improved significantly, and systolic blood pressure was decreased significantly (P<0.01). MCP-1, CCR-2 protein and mRNA expression in thoracic aorta of benazepril and mangiferin groups decreased significantly (P<0.01). Conclusion:There are inflammation damages in carotid artery, thoracic aorta and superior mesenteric artery of spontaneously hypertensive rats. Mangiferin has an anti-inflammatory effect by possibly inhibiting the expressions of MCP-1/CCR-2 pathway in SHR vessels.
